Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:31608
Zwart R, et al., The restricted expression pattern of the POU factor Oct-6 during early development of the mouse nervous system. Mech Dev. 1996 Feb;54(2):185-94
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:5441502
|
Gene symbol:
|
Pou3f1
|
Gene name:
|
POU domain, class 3, transcription factor 1
|
Antibody:
|
anti-Oct-6
|
Detection system:
|
Secondary antibody coupled to alkaline phosphatase
|
|
Results
|
Specimen
3A:
embryonic day 7.0
(more )
Note: Late-streak stage embryo.
Specimen
3A:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Late-streak stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo ectoderm |
Present |
Regionally restricted |
3A
|
Expression is present in the anterior half the embryo. It is not found in the posterior and lateral-posterior parts of the embryo.
|
TS10: extraembryonic ectoderm |
Present |
Regionally restricted |
3A
|
Expression is present in the ectodermal component of the chorion membrane.
|
Specimen
3B:
embryonic day 7.0
(more )
Note: Early bud stage embryo.
Specimen
3B:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Early bud stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo ectoderm |
Present |
Regionally restricted |
3B
|
Expression is present in the anterior region.
|
TS10: extraembryonic ectoderm |
Present |
Regionally restricted |
3B
|
Expression is present in the ectodermal component of the chorion membrane.
|
Specimen
3C:
embryonic day 7.5
(more )
Note: Advanced headfold stage embryo.
Specimen
3C:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 7.5
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Advanced headfold stage embryo.
|
Specimen
3D:
embryonic day 7.0
(more )
Note: No-bud, neural plate stage embryo.
Specimen
3D:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
No-bud, neural plate stage embryo.
|
Specimen
3F:
embryonic day 7.0
(more )
Note: No-bud, neural plate stage embryo.
Specimen
3F:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 7.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
No-bud, neural plate stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS10: embryo ectoderm |
Present |
Regionally restricted |
3F
|
Expression is present in the anterior region. There is a stripe of non-expressing cells at the midline of the embryo anterior of the node.
|
TS10: extraembryonic ectoderm |
Present |
Regionally restricted |
3F
|
Expression is present in the ectodermal component of the chorion membrane.
|
Specimen
4A:
embryonic day 8.0
(more )
Note: 5-somite stage embryo.
Specimen
4A:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
5-somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: future brain neural fold |
Strong |
Uniform |
4A
|
There is strong expression in the head folds. It is evenly distributed along the entire medial to lateral axis of the headfolds.
|
Specimen
4C:
embryonic day 9.0
(more )
Note: 17-somite stage embryo.
Specimen
4C:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
17-somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: future midbrain |
Present |
|
4C
|
The lateral and anterior boundaries of expression are regressing, leading to a more ventral position of expression.
|
TS14: prosencephalon |
Present |
|
4C
|
The lateral and anterior boundaries of expression are regressing, leading to a more ventral position of expression.
|
TS14: future hindbrain |
Present |
Regionally restricted |
4C
|
Expression regresses in the hindbrain region in a ventral and anterior direction.
|
Specimen
4D:
embryonic day 9.0
(more )
Note: 17-somite stage embryo.
Specimen
4D:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 9.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
17-somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS14: rhombomere 1 |
Present |
|
4D
|
The posterior boundary of expression is located rostral of rhombomere 6.
|
TS14: rhombomere 2 |
Present |
|
4D
|
The posterior boundary of expression is located rostral of rhombomere 6.
|
TS14: rhombomere 3 |
Present |
|
4D
|
The posterior boundary of expression is located rostral of rhombomere 6.
|
TS14: rhombomere 4 |
Present |
|
4D
|
The posterior boundary of expression is located rostral of rhombomere 6.
|
TS14: rhombomere 5 |
Present |
|
4D
|
The posterior boundary of expression is located rostral of rhombomere 6.
|
TS14: rhombomere 6 |
Absent |
|
4D
|
The posterior boundary of expression is located rostral of rhombomere 6.
|
Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: future midbrain |
Present |
Regionally restricted |
5A
|
Expression is present in the ventral aspect of the rostral mesencephalon. Expression is also seen in a number of neurons in a dorsal position.
|
TS15: diencephalon |
Present |
Regionally restricted |
5A
|
Expression is present in the caudal diencephalon.
|
TS15: telencephalon |
Present |
Regionally restricted |
5A
|
Expression is present at the ventral midline of the secondary prosencephalon. Expression is also seen in a number of neurons in a dorsal position.
|
TS15: future hindbrain |
Absent |
|
5A
|
|
|