Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:31608
Zwart R, et al., The restricted expression pattern of the POU factor Oct-6 during early development of the mouse nervous system. Mech Dev. 1996 Feb;54(2):185-94
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:5441501
|
Gene symbol:
|
Pou3f1
|
Gene name:
|
POU domain, class 3, transcription factor 1
|
Probe:
|
Oct-6 cDNA
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
6A:
embryonic day 9.5
(more )
Specimen
6A:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Present |
Regionally restricted |
6A
|
Expression is present in the basal segments of prosomeres 1 and 2.
|
TS15: telencephalon |
Present |
Regionally restricted |
6A
|
Expression is present in the ventral part of prosomere 6 of the secondary prosencephalon.
|
TS15: future midbrain |
Present |
|
6A
|
|
Specimen
6B:
embryonic day 9.5
(more )
Specimen
6B:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Present |
Regionally restricted |
6B
|
Expression is present at the ventral midline of the rostral diencephalon.
|
Specimen
6C:
embryonic day 9.5
(more )
Note: Double labeled: blue - Pou3f1; red/brown - Shh (assay MGI:5441500).
Specimen
6C:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Note:
|
Double labeled: blue - Pou3f1; red/brown - Shh (assay MGI:5441500).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Present |
Regionally restricted |
6C
|
Expression is present in the basal segments of prosomeres 1 and 2.
|
TS15: telencephalon |
Present |
Regionally restricted |
6C
|
Expression is present in the ventral part of prosomere 6 of the secondary prosencephalon.
|
TS15: future midbrain |
Present |
|
6C
|
|
Specimen
6D:
embryonic day 9.5
(more )
Note: Double labeled: blue - Pou3f1; red/brown - Shh (assay MGI:5441500).
Specimen
6D:
(close )
Genetic Background:
|
involves: C57BL/10 * CBA
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: blue - Pou3f1; red/brown - Shh (assay MGI:5441500).
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon |
Present |
Regionally restricted |
6D
|
Expression is present at the ventral midline of the rostral diencephalon.
|
|