Antibody:	anti-Oct-6
Detection system:	Secondary antibody coupled to fluorescein
Assay notes:	Embryos were fixed in 35% acetone, 35% methanol, 5% acetic acid, and 25% water solution.

Structure	Level	Pattern	Image	Note
TS4: blastocyst	Absent

Structure	Level	Pattern	Image	Note
TS7: epiblast	Present		2	Expression is present in the embryonic ectoderm.
TS7: extraembryonic ectoderm	Present		2

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present		3E	The posterior boundary of expression extends to the distal tip of the embryo. This position coincides with the position of the node.
TS10: extraembryonic ectoderm	Present	Regionally restricted	3E	Expression is present in the ectodermal component of the chorion membrane.
TS10: primitive streak	Absent		3E	There is no expression in the midsagittal streak.
TS10: embryo endoderm	Absent		3E

Structure	Level	Pattern	Image	Note
TS12: future brain neural fold	Strong		4B	Expression extends to the anterior extremity of the neuroectoderm.

Structure	Level	Pattern	Image	Note
TS15: diencephalon	Present	Regionally restricted	5B	Expression is present in the basal plate.
TS15: future midbrain	Present	Regionally restricted	5B	Expression is present in the basal plate.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	5C	Expression is restricted rostral of the infundibular recess and caudal of the optic chiasm.
TS15: Rathke's pouch	Absent		5C
TS15: infundibular recess of 3rd ventricle	Absent		5C

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present	Regionally restricted	5D	Expression is restricted rostral of the infundibular recess and caudal of the optic chiasm.
TS15: Rathke's pouch	Absent		5D
TS15: infundibular recess of 3rd ventricle	Absent		5D