Probe:	Limk1 cDNA5
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Absent		3A	Neither the epiblast nor the primitive endodermal lineages express transcripts.
TS9: embryo endoderm	Absent		3A	Neither the epiblast nor the primitive endodermal lineages express transcripts.
TS9: primary trophoblast giant cell	Strong		3A

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		3A

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		3B
TS12: future midbrain	Present		3B
TS12: future prosencephalon	Present		3B
TS12: heart	Present		3B
TS12: hindgut diverticulum	Present		3B
TS12: cardiac muscle tissue	Strong		3B	Highest levels of hybridization in the heart were in the myocardial tissue.
TS12: primitive heart tube endocardial tube	Weak		3B

Structure	Level	Pattern	Image	Note
TS12: future hindbrain	Present		3C
TS12: future prosencephalon	Present		3C
TS12: embryo mesenchyme	Present	Widespread	3C
TS12: head mesenchyme	Weak	Widespread	3C
TS12: neural tube	Absent		3C	No expression was apparent in more caudal levels of the neural tube.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	3D	Expression was localized in the cells surrounding the mesencephalic vesicle.
TS15: 1st branchial arch	Present		3D
TS15: 2nd branchial arch	Present		3D
TS15: hindgut	Present		3D
TS15: umbilical vein	Present		3D
TS15: future brain	Present	Regionally restricted	3D	Expression was detected in the ventro-medial areas of the developing brain.

Structure	Level	Pattern	Image	Note
TS12: future prosencephalon	Present		4A
TS12: neural fold	Present		4A
TS12: head	Present	Regionally restricted	4A	Expression was detected in the head folds.
TS12: foregut diverticulum	Present	Regionally restricted	4A	Expression was detected in the anterior foregut.

Structure	Level	Pattern	Image	Note
TS12: allantois mesenchyme	Present	Regionally restricted	4B	Expression was detected in mesodermal cells at the base of the allantois.
TS12: hindgut diverticulum	Present	Regionally restricted	4B	Expression was detected in the midline and lateral regions of the hindgut.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain	Present		4C
TS13: future midbrain	Present		4C
TS13: future prosencephalon	Present		4C
TS13: hindgut diverticulum	Present		4C

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Present		4D
TS15: future hindbrain	Present		4D
TS15: future midbrain	Present		4D
TS15: telencephalon	Present	Regionally restricted	4D	Expression was detected around the telencephalic vesicle.
TS15: nasal process	Present	Regionally restricted	4D	Expression was detected in the lateral nasal process.
TS15: hindgut	Present		4D
TS15: olfactory placode	Present		4D

Structure	Level	Pattern	Image	Note
TS15: foregut	Present		4E
TS15: future forebrain	Present		4E
TS15: future hindbrain	Present		4E
TS15: future midbrain	Present		4E
TS15: hindgut	Present		4E

Structure	Level	Pattern	Image	Note
TS11: ectoplacental cone	Weak	Regionally restricted	5A	Expression was detected in the diploid cell population.
TS11: polar trophectoderm	Present	Regionally restricted	5A	Intense labeling was detected in the secondary giant cells forming at the margins of the ectoplacental cone.

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Strong		5B	Strong expression was detected in secondary giant cells overlying the developing placenta.
TS15: allantois	Weak		5B
TS15: spongiotrophoblast	Weak		5B
TS15: chorioallantoic placenta	Weak	Regionally restricted	5B	Lower levels of expression were uniformly present within the diploid cells of the rudimentary chorioallantoic placenta.

Structure	Level	Pattern	Image	Note
TS28: decidua	Weak		5B

Structure	Level	Pattern	Image	Note
TS15: trophoblast giant cell	Strong	Regionally restricted	5C	Expression was strong in secondary giant cells overlying the visceral yolk sac.