Antibody:	anti-neurofilament clone 2H3
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		5A left
TS15: central nervous system	Present	Regionally restricted	5A left	Authors report expression in cranial amd spinal nerves.
TS15: spinal component of peripheral nervous system	Present	Regionally restricted	5A left	Authors report expression in cranial amd spinal nerves.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		5A right	Expression in rudimentary cranial cranglia.
TS15: glossopharyngeal preganglion	Present		5A right	Authors report defective formation of superior regions of the glossopharyngeal ganglion, superior vagal ganglion and cranial accessory neurons.
TS15: vagus preganglion	Present		5A right	Authors report defective formation of superior regions of the glossopharyngeal ganglion, superior vagal ganglion and cranial accessory neurons.
TS15: central nervous system	Present	Regionally restricted	5A right	No detectable cranial nerve development.
TS15: spinal component of peripheral nervous system	Absent		5A right	No detectable spinal nerve development.

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present		5B left
TS17: cranial nerve	Present		5B left
TS17: spinal nerve	Present		5B left

Structure	Level	Pattern	Image	Note
TS17: cranial ganglion	Present		5B right
TS17: glossopharyngeal ganglion	Present		5B right	Authors state that superior regions of the glossopharyngeal ganglion, superior vagal ganglion and cranial accessory neurons are developmentally arrested.
TS17: vagus ganglion	Present		5B right	Authors state that superior regions of the glossopharyngeal ganglion, superior vagal ganglion and cranial accessory neurons are developmentally arrested.
TS17: cranial nerve	Absent		5B right	No detectable spinal or cranial nerve development.
TS17: spinal nerve	Absent		5B right	No detectable spinal or cranial nerve development.