Probe:	5sg8
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: future brain	Present		3B/E	Expressed near the midbrain-hindbrain boundary. The signal was weaker than those at later stages.
TS15: midbrain-hindbrain junction	Present		3B/E	Expressed near the midbrain-hindbrain boundary. The signal was weaker than those at later stages.
TS15: otocyst	Absent		3B/E	No expression in the otic vesicles.
TS15: nephric duct	Absent		3B/E	No expression in the pronephric duct.
TS15: mesonephric tubule	Absent		3B/E	No expression in the pronephric tubules.

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present		3C/F
TS19: metencephalon	Present		3C/F
TS19: myelencephalon	Weak		3C/F

Structure	Level	Pattern	Image	Note
TS19: cerebellum primordium	Present		3D/G
TS19: metencephalon basal plate	Present		3D/G	Expressed in pons.
TS19: midbrain	Present	Regionally restricted	3D/G	Expressed in the posterior part of tegmentum, which is the ventral part of mesencephalon.

Structure	Level	Pattern	Image	Note
TS19: midbrain	Present	Regionally restricted	4A/D	Expressed in the tegmentum, which is the ventral part of mesencephalon.
TS19: metencephalon	Present		4A/D

Structure	Level	Pattern	Image	Note
TS19: metencephalon	Present	Regionally restricted	4B/E	Expressed in the metencephalon as a complete ring. Expressed not only in the ventricular zone, but also in the intermediate zone.
TS19: metencephalon ventricular layer	Present		4B/E

Structure	Level	Pattern	Image	Note
TS19: myelencephalon lateral wall	Weak	Regionally restricted	4C/F	Expression is restricted to the medial regions.
TS19: neural retinal epithelium	Absent		4C/F
TS19: optic stalk	Absent		4C/F

Structure	Level	Pattern	Image	Note
TS21: metencephalon	Present	Regionally restricted	5C/F	Expression extended through the rhombic isthmus, the boundary between the metencephalon and mesencephalon.
TS21: midbrain-hindbrain junction	Present	Regionally restricted	5C/F	Expression extended through the rhombic isthmus, the boundary between the metencephalon and mesencephalon.
TS21: midbrain	Present	Regionally restricted	5C/F	Expressed in posterior half of tegmentum.

Structure	Level	Pattern	Image	Note
TS21: metencephalon	Present	Regionally restricted	5D/G	Expression extended through the rhombic isthmus, the boundary between the metencephalon and mesencephalon.
TS21: midbrain-hindbrain junction	Present	Regionally restricted	5D/G	Expression extended through the rhombic isthmus, the boundary between the metencephalon and mesencephalon.
TS21: midbrain	Present	Regionally restricted	5D/G	Expressed in posterior half of tegmentum.

Structure	Level	Pattern	Image	Note
TS23: midbrain-hindbrain junction	Present	Regionally restricted	5E/H	Expressed in the midbrain-hindbrain boundary, in the region between the cerebellum and the inferior colliculus.
TS23: midbrain	Present	Regionally restricted	5E/H	Expressed in the mesencephalic flexure.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Widespread	6A/B	Expressed along the entire length of the neural tube.

Structure	Level	Pattern	Image	Note
TS19: neural tube ventricular layer	Present		6C/F
TS19: neural tube lateral wall	Present	Regionally restricted	6C/F	Expressed in the lateral margin of the ventricular zone of both alar and basal plates and also in the intermediate zone.

Structure	Level	Pattern	Image	Note
TS21: metanephros	Absent		6D/G

Structure	Level	Pattern	Image	Note
TS19: optic stalk	Absent		6E/H
TS19: inner ear	Absent		6E/H	No expression in the otic vesicle.

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Present	Widespread		Expressed along the entire length of the neural tube.