Gene Expression Data
Assay Details
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Assay
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Reference:
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J:19219
Kostic D, et al., Targeted disruptions of the murine Hoxa-4 and Hoxa-6 genes result in homeotic transformations of components of the vertebral column. Mech Dev. 1994 Jun;46(3):231-47
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5441250
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Gene symbol:
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Nefm
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Gene name:
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neurofilament, medium polypeptide
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Antibody:
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anti-neurofilament clone 2H3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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Fixative: 3.5% paraformaldehyde.
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Results
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Specimen
not shown:
embryonic day 10.5
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Specimen
not shown:
embryonic day 10.5; Hoxa4tm1Mrc/Hoxa4 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxa4tm1Mrc/Hoxa4+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxa4tm1Mrc/Hoxa4tm1Mrc
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cranial nerve |
Present |
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No noticeable difference was observed in the morphology of the cranial nerves between the wild type and mutant embryos.
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Specimen
not shown:
embryonic day 10.5; Hoxa6tm1Mrc/Hoxa6 +
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxa6tm1Mrc/Hoxa6+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxa6tm1Mrc/Hoxa6tm1Mrc
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cranial nerve |
Present |
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No noticeable difference was observed in the morphology of the cranial nerves between the wild type and mutant embryos.
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Specimen
8A:
postnatal newborn
(more )
Note: Patches of enteric wall from the cecum and colon were collected.
Specimen
8A:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Note:
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Patches of enteric wall from the cecum and colon were collected.
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Specimen
8B:
postnatal newborn; Hoxa4tm1Mrc/ Hoxa4tm1Mrc
(more )
Note: Patches of enteric wall from the cecum and colon were collected.
Specimen
8B:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxa4tm1Mrc/Hoxa4tm1Mrc
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Note:
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Patches of enteric wall from the cecum and colon were collected.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: enteric ganglion |
Present |
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8B
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None of the regions examined showed any differences between the wild type and mutant, either in the density of the ganglia or in the number of cells per ganglion.
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Specimen
not shown:
postnatal adult
(more )
Note: Patches of enteric wall from the cecum and colon were collected.
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Note:
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Patches of enteric wall from the cecum and colon were collected.
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Specimen
not shown:
postnatal adult; Hoxa4tm1Mrc/ Hoxa4tm1Mrc
(more )
Note: Patches of enteric wall from the cecum and colon were collected.
Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S7/SvEvBrd * C57BL/6J
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Mutant Allele(s):
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Hoxa4tm1Mrc/Hoxa4tm1Mrc
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Paraformaldehyde
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Note:
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Patches of enteric wall from the cecum and colon were collected.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: enteric ganglion |
Present |
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None of the regions examined showed any differences between the wild type and mutant, either in the density of the ganglia or in the number of cells per ganglion.
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