Hoxa6 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:19219 Kostic D, et al., Targeted disruptions of the murine Hoxa-4 and Hoxa-6 genes result in homeotic transformations of components of the vertebral column. Mech Dev. 1994 Jun;46(3):231-47
Assay type:	RT-PCR
MGI Accession ID:	MGI:5441249
Gene symbol:	Hoxa6
Gene name:	homeobox A6

Probe:	Hoxa6-pE, Hoxa6-pF
Probe preparation:	DNA
Assay notes:	The RT-PCR reaction was resolved on agarose gel, transferred to nylon membrane and hybridized with an oligo probe (MGI:5441246) that was internal to the primers used for amplification.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	260 bp	Amount	Genetic Background	Mutant Allele(s)	Sex
+/+	E12.5	TS20: embryo	Present	5 µg; total RNA	Not Specified		Not Specified
+/-	E12.5	TS20: embryo	Present (a)	5 µg; total RNA	involves: 129S7/SvEvBrd * C57BL/6J	Hoxa6^tm1Mrc/Hoxa6⁺	Not Specified
-/-	E12.5	TS20: embryo	Absent (a)	5 µg; total RNA	involves: 129S7/SvEvBrd * C57BL/6J	Hoxa6^tm1Mrc/Hoxa6^tm1Mrc	Not Specified

Notes:
(a) While the 260bp band was not detected in the homozygous mutants, further amplification detected a 1.4kb band. It was identified as a readthrough transcript in the -/- embryos by probing for neo. It was barely detectable in +/- embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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