Antibody:	anti Pax2 Z-RX2 (71-6000)
Detection system:	Secondary antibody coupled to rhodamine

Structure	Level	Pattern	Image	Note
TS18: optic stalk	Present	Regionally restricted	6A	Expressed in the ventral part of the optic stalk.
TS18: retina	Present	Regionally restricted	6A	Expressed in the part of the neural retina farthest from the surface ectoderm.
TS18: lens pit	Absent		6A

Structure	Level	Pattern	Image	Note
TS18: myelencephalon lateral wall	Present	Regionally restricted	6B	Expressing cells are distal to the lumen, tightly clustered, and adjacent to the processes of the marginal layer.

Structure	Level	Pattern	Image	Note
TS18: neural tube lateral wall	Present	Regionally restricted	6C	Expressing cells are tightly clustered in two large domains dorsal and ventral to the sulcus limitans. They form two longitudinal columns that extend along all but the most caudal part of the neural tube.
TS18: dorsal root ganglion	Absent		6C
TS18: neural tube floor plate	Absent		6C

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Regionally restricted	6D , 6E	Expressed in cells lies directly lateral to the neuroepithelium and extends dorso-ventrally, excluding the most ventral quarter of the neural tube.
TS20: spinal cord lateral wall	Present	Regionally restricted	6E , 6D	The two more medial compartments, previously delineated by the sulcus limitans, have merged into one continuous region.
TS20: spinal cord marginal layer	Present	Regionally restricted	6E	Expressed in the cells, presumably neurons that have left the mitotic cycle sooner and migrated radially, located adjacent to the axon bundles in the marginal layer.

Structure	Level	Pattern	Image	Note
TS22: spinal cord	Present	Diffuse	6F , 6G	Expressing cells are dispersed throughout the neural tube with a high concentration in the intermediate grey region of the pars dorsalis and pars ventralis. Also expressed in the most dorsal region of the neural tube.
TS22: spinal cord marginal layer	Present	Diffuse	6G	Expressing cells are located adjacent to, but not within the axon bundles of the marginal layer.

Structure	Level	Pattern	Image	Note
TS25: spinal cord	Present			The positions of the expressing cells are similar as at E14. Expression begins to decrease at E17, the intensity of staining is lower although the number of positive cells is high.