Gene Expression Data
Assay Details
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Assay
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Reference:
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J:98114
Friedman RA, et al., Eya1 acts upstream of Tbx1, Neurogenin 1, NeuroD and the neurotrophins BDNF and NT-3 during inner ear development. Mech Dev. 2005 May;122(5):625-34
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5441099
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Gene symbol:
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Ntf3
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Gene name:
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neurotrophin 3
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Probe:
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NT-3 probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5A:
embryonic day 10.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Present |
Regionally restricted |
5A
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Specimen
5B:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: otocyst |
Weak |
Regionally restricted |
5B
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Reduced expression in the ventrolateral domain compared to wild type embryos.
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Specimen
5C:
embryonic day 12.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlea |
Present |
Regionally restricted |
5C
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Expression in the developing cochlear epithelium.
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Specimen
5D:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlea |
Weak |
Regionally restricted |
5D
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Reduced expression in ventral structures at the level of the sacculus. Little or no expression in the developing cochlear epithelium.
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Specimen
5E:
embryonic day 12.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlea |
Present |
Regionally restricted |
5E
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Expression in the developing cochlear epithelium.
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Specimen
5F:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlea |
Weak |
Regionally restricted |
5F
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Reduced expression in ventral structures at the level of the cochlear base. Little or no expression in the developing cochlear epithelium.
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Specimen
5G:
embryonic day 12.5
(more )
Specimen
5G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlea |
Present |
Regionally restricted |
5G
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Expression in the developing cochlear epithelium.
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Specimen
5H:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cochlea |
Weak |
Regionally restricted |
5H
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Reduced expression in ventral structures at the cochlear hook region. Little or no expression in the developing cochlear epithelium.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: C3HeB/FeJ
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Mutant Allele(s):
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Eya1bor/Eya1bor
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Trace |
Regionally restricted |
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Authors report greatly reduced expression in the cochlear epithelium.
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Analysis Tools