Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:23131
Conlon FL, et al., Effects of the TWis mutation on notochord formation and mesodermal patterning. Mech Dev. 1995 Feb;49(3):201-9
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5441038
|
|
Gene symbol:
|
Meox1
|
|
Gene name:
|
mesenchyme homeobox 1
|
|
|
Results
|
Specimen
2A:
embryonic day 7.5
(more  )
Note: Late head fold stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 7.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
Late head fold stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS11: head mesenchyme |
Present |
Regionally restricted |
2A
|
Expression is present in lateral strips at the base of the hindbrain.
|
Specimen
2B:
embryonic day 8.5
(more )
Note: 9 somite stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
9 somite stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
|
Specimen
2C:
embryonic day 9.0
(more )
Note: 16 somite stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
Specimen
2C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
16 somite stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
|
Specimen
2D:
embryonic day 9.0
(more )
Note: 16 somite stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
Specimen
2D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Embedding:
|
Paraffin
|
|
Note:
|
16 somite stage embryo. Double labeled: blue - Meox1; brown - T (assay MGI:5441039).
|
Specimen
Not shown:
embryonic day 8.0
(more )
Note: 1 2 somite stage.
Specimen
Not shown:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
1 2 somite stage.
|
Specimen
Not shown:
embryonic day 8.0; TWis/ TWis
(more )
Note: 1-2 somite stage equivalent.
Specimen
Not shown:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Mutant Allele(s):
|
TWis/TWis
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
1-2 somite stage equivalent.
|
Specimen
Not shown:
embryonic day 8.0
(more )
Note: 3-4 somite stage.
Specimen
Not shown:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
3-4 somite stage.
|
Specimen
Not shown:
embryonic day 8.0; TWis/ TWis
(more )
Note: 3-4 somite stage equivalent.
Specimen
Not shown:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Mutant Allele(s):
|
TWis/TWis
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
3-4 somite stage equivalent.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: paraxial mesenchyme |
Present |
Regionally restricted |
|
Relative to wild type, expression is decreasing in more caudal regions of the embryo.
|
Specimen
3A:
embryonic day 9.0; TWis/ TWis
(more )
Note: This embryo corresponds to a 13-somite stage embryo.
Specimen
3A:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Mutant Allele(s):
|
TWis/TWis
|
|
Age:
|
embryonic day 9.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
This embryo corresponds to a 13-somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: paraxial mesenchyme |
Present |
Regionally restricted |
3A
|
Expression is no longer present in the posterior paraxial or somitic mesoderm.
|
Specimen
4B:
embryonic day 8.5
(more )
Note: 12-somite stage embryo. Double labeled: brown - Meox1; blue - Twist1 (assay MGI:5441040).
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
12-somite stage embryo. Double labeled: brown - Meox1; blue - Twist1 (assay MGI:5441040).
|
Specimen
4C:
embryonic day 8.5
(more )
Note: 12-somite stage embryo. Double labeled: brown - Meox1; blue - Twist1 (assay MGI:5441040).
Specimen
4C:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Age:
|
embryonic day 8.5
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Embedding:
|
Paraffin
|
|
Note:
|
12-somite stage embryo. Double labeled: brown - Meox1; blue - Twist1 (assay MGI:5441040).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: somite |
Present |
Regionally restricted |
4C
|
Expression is present in the sclerotome.
|
Specimen
Not shown:
embryonic day 10.0; TWis/ TWis
(more )
Note: 20-22 somite stage embryo.
Specimen
Not shown:
(close )
|
Genetic Background:
|
involves: 129/SvEv
|
|
Mutant Allele(s):
|
TWis/TWis
|
|
Age:
|
embryonic day 10.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Note:
|
20-22 somite stage embryo.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: trunk paraxial mesenchyme |
Present |
Regionally restricted |
|
Morphologically distinct somites do not form in the mutants. Expression is never obseved in more than eight paired "blocks" at this stage.
|
|
Analysis Tools