Probe:	Shh cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1C	Expression was confined to the ventral midline of the neural tube.
TS13: hindgut diverticulum	Present		1C

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1F	Expression was detected in the ventral midline.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	1I	Expression was detected along the neural tube.
TS15: 1st branchial arch mandibular component	Present		1I

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch endoderm	Present		1L
TS17: brain	Present		1L
TS17: floor plate	Present		1L
TS17: limb	Present		1L

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Weak	Regionally restricted	2C	Expression was detected in the posterior half of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	2F	Expression was detected in the posterior half of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Present	Regionally restricted	2I	Expression in the posterior half of the hindlimb bud has expanded.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Regionally restricted	4C	Expression was detected along the ventral midline of the hindbrain and midbrain.
TS20: midbrain	Present	Regionally restricted	4C	Expression was detected along the ventral midline of the hindbrain and midbrain.
TS20: thalamus	Present	Regionally restricted	4C	Expression was detected in the zona limitans.
TS20: hypothalamus	Present		4C
TS20: mandibular process	Present		4C
TS20: medial ganglionic eminence	Present	Regionally restricted	4C	Expression was detected in the medial face of the medial ganglionic eminence.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	4K	Expression was detected in the midline.
TS19: hindbrain	Present	Regionally restricted	4K	Expression was detected in the midline.
TS19: midbrain	Present	Regionally restricted	4K	Expression was detected in the midline.

Structure	Level	Pattern	Image	Note
TS21: gut	Present	Regionally restricted	5C	Expression was detected in the endoderm.

Structure	Level	Pattern	Image	Note
TS20: vibrissa follicle	Present	Regionally restricted	5F	Expression was detected in the central ectodermal cells.