Probe:	Gli cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1A	Expression was detected in a broad domain along the neural tube.
TS13: hindgut diverticulum	Present		1A
TS13: lateral plate mesoderm	Present		1A
TS13: somite	Present	Regionally restricted	1A	Expression was detected in the ventral somites.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1D	Expression was detected in a ventral domain.

Structure	Level	Pattern	Image	Note
TS15: central nervous system	Present	Regionally restricted	1G	Expression was detected along the neural tube.
TS15: 1st branchial arch mandibular component	Strong		1G

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present		1J
TS17: sclerotome	Present		1J
TS17: central nervous system	Present	Regionally restricted	1J	Expression flanks the floor plate where Shh is expressed. Expression is strongest at the posterior end.
TS17: limb	Present	Regionally restricted	1J	Expression was detected in the posterior half of each limb bud.
TS17: forebrain	Present	Regionally restricted	1J	Expression was confined predominantly to cells flanking the ventral midline.
TS17: midbrain	Present	Regionally restricted	1J	Expression was confined predominantly to cells flanking the ventral midline.
TS17: diencephalon	Present	Regionally restricted	1J	A dorsal stripe of expression was observed.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud mesenchyme	Present	Regionally restricted	2A	Expression was detected in the posterior half of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud mesenchyme	Present	Regionally restricted	2D	Expression was detected in the posterior half of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud mesenchyme	Absent		2G	Expression has faded from the most posterior region.

Structure	Level	Pattern	Image	Note
TS20: forelimb digit 2 mesenchyme	Present	Regionally restricted	3A	Expression was detected in the condensing mesenchyme.
TS20: forelimb digit 3 mesenchyme	Present	Regionally restricted	3A	Expression was detected in the condensing mesenchyme.
TS20: forelimb digit 4 mesenchyme	Present	Regionally restricted	3A	Expression was detected in the condensing mesenchyme.
TS20: radius-ulna pre-cartilage condensation	Present	Regionally restricted	3A	Expression was detected in the condensing mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit 2 phalanx pre-cartilage condensation	Strong	Regionally restricted	3E	Expression was strong in the perichondrium of the most proximal phalanges of digits 4, 3, and 2.
TS21: hindlimb digit 3 phalanx pre-cartilage condensation	Strong	Regionally restricted	3E	Expression was strong in the perichondrium of the most proximal phalanges of digits 4, 3, and 2.
TS21: hindlimb digit 4 phalanx pre-cartilage condensation	Strong	Regionally restricted	3E	Expression was strong in the perichondrium of the most proximal phalanges of digits 4, 3, and 2.
TS21: hindlimb digit 1 phalanx pre-cartilage condensation	Weak	Regionally restricted	3E	Expression was weak in the perichondrium of the most proximal phalanges of digits 5 and 1.
TS21: hindlimb digit 5 phalanx pre-cartilage condensation	Weak	Regionally restricted	3E	Expression was weak in the perichondrium of the most proximal phalanges of digits 5 and 1.
TS21: hindlimb digit	Weak	Regionally restricted	3E	Expression was weak at the distal tips of the early digits.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit 2 phalanx pre-cartilage condensation	Strong	Regionally restricted	3G	Expression was strong in the perichondrium of the most proximal phalanges of digits 4, 3, and 2.
TS21: hindlimb digit 3 phalanx pre-cartilage condensation	Strong	Regionally restricted	3G	Expression was strong in the perichondrium of the most proximal phalanges of digits 4, 3, and 2.
TS21: hindlimb digit 4 phalanx pre-cartilage condensation	Strong	Regionally restricted	3G	Expression was strong in the perichondrium of the most proximal phalanges of digits 4, 3, and 2.
TS21: hindlimb digit 1 phalanx pre-cartilage condensation	Weak	Regionally restricted	3G	Expression was weak in the perichondrium of the most proximal phalanges of digits 5 and 1.
TS21: hindlimb digit 5 phalanx pre-cartilage condensation	Weak	Regionally restricted	3G	Expression was weak in the perichondrium of the most proximal phalanges of digits 5 and 1.
TS21: hindlimb digit	Weak	Regionally restricted	3G	Expression was weak at the distal tips of the early digits.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit 2 phalanx pre-cartilage condensation	Present	Regionally restricted	3I	Expression was strong over the first two phalanges and weak over the third phalange of digits 4, 3 and 2.
TS21: forelimb digit 3 phalanx pre-cartilage condensation	Present	Regionally restricted	3I	Expression was strong over the first two phalanges and weak over the third phalange of digits 4, 3 and 2.
TS21: forelimb digit 4 phalanx pre-cartilage condensation	Present	Regionally restricted	3I	Expression was strong over the first two phalanges and weak over the third phalange of digits 4, 3 and 2.
TS21: forelimb digit 5 phalanx pre-cartilage condensation	Present	Regionally restricted	3I	Expression was found only in the first two phalanges of digit 5.
TS21: forelimb digit 1 phalanx pre-cartilage condensation	Present	Regionally restricted	3I	Expression was found only in the first phalange of digit 1.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit cartilage condensation	Present	Regionally restricted	3M	Expression marks all the phalanges.
TS22: hindlimb digit 1 phalanx cartilage condensation	Present	Regionally restricted	3M	Expression was detected in second phalange of digit 1.
TS22: hindlimb digit 2 phalanx cartilage condensation	Present	Regionally restricted	3M	Expression was detected in the second phalanges of digits 4, 3 and 2. The expression was more broad than in digit 1.
TS22: hindlimb digit 3 phalanx cartilage condensation	Present	Regionally restricted	3M	Expression was detected in the second phalanges of digits 4, 3 and 2. The expression was more broad than in digit 1.
TS22: hindlimb digit 4 phalanx cartilage condensation	Present	Regionally restricted	3M	Expression was detected in the second phalanges of digits 4, 3 and 2. The expression was more broad than in digit 1.

Structure	Level	Pattern	Image	Note
TS20: forebrain	Present	Regionally restricted	4A	Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
TS20: hindbrain	Present	Regionally restricted	4A	Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
TS20: midbrain	Present	Regionally restricted	4A	Expression was observed in ventral longitudinal stripes which extend along the hindbrain, midbrain, and continue into the ventral forebrain. The longitudinal stripes branch off to a region surrounding the zone limitans intrathalamica.
TS20: mandibular process	Present		4A

Structure	Level	Pattern	Image	Note
TS21: gut mesenchyme	Present	Regionally restricted	5A	Expression was detected in the mesoderm in a ring around Shh-expressing cells in the endoderm.

Structure	Level	Pattern	Image	Note
TS20: vibrissa mesenchyme	Present	Regionally restricted	5D	Expression was detected in the mesoderm in a ring around the central ectodermal cells expressing Shh.

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present		6A

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	6B	Ectopic expression was detected in an anterior domain.