Probe:	pceck2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	4A	Expression was detected in regions adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	4B	Expression was detected at or near the distal region of the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: embryo	Present	Regionally restricted	4C	Expression was detected in regions adjacent to the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	4D	Expression was detected at or near the distal region of the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	4E	Section from the posterior boundary of the expression domain. Expression was detected in ectodermal cells flanking the primitive streak.
TS10: embryo endoderm	Absent		4E
TS10: embryo mesoderm	Absent		4E

Structure	Level	Pattern	Image	Note
TS10: embryo ectoderm	Present	Regionally restricted	4F	Section from the distal end of the embryo at the anterior boundary of the expression domain. Expression was detected in ectodermal cells adjacent to the primitive streak.
TS10: embryo mesoderm	Present		4F

Structure	Level	Pattern	Image	Note
TS11: primitive streak	Present	Regionally restricted	4G	Expression was detected in the distal region.
TS11: embryo ectoderm	Present		4G
TS11: embryo mesoderm	Present		4G

Structure	Level	Pattern	Image	Note
TS11: node	Present	Regionally restricted	4H	Expression was in a population of cells in the extreme distal end or node of the streak.

Structure	Level	Pattern	Image	Note
TS11: node	Present		5A

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	5B	Expression was detected in the distal head fold region.

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Regionally restricted	5C	Expression was detected in a narrow band of approximately 5 cells that extends the entire width of the head folds but does not include the neural ectoderm.
TS11: neural ectoderm	Absent		5C

Structure	Level	Pattern	Image	Note
TS11: embryo mesoderm	Present		5D

Structure	Level	Pattern	Image	Note
TS12: embryo mesenchyme	Present	Regionally restricted	5E	Expression lies caudal to rhombomere 3.
TS12: neural plate	Present	Regionally restricted	5E	Expression lies caudal to rhombomere 3.

Structure	Level	Pattern	Image	Note
TS12: node	Present		5G

Structure	Level	Pattern	Image	Note
TS12: neural plate	Present		5H

Structure	Level	Pattern	Image	Note
TS12: node	Present		6A
TS12: rhombomere	Present	Regionally restricted	6A	Expression was detected in prospective rhombomere 4.
TS12: head mesenchyme	Present	Regionally restricted	6A	Expression was detected in mesenchymal cells underlying rhombomere 4 and 5.

Structure	Level	Pattern	Image	Note
TS12: rhombomere	Present	Regionally restricted	6D	In rhombomere 4, high expression was detected in the dorsal aspect of the neural epithelium.
TS12: lateral plate mesoderm	Present		6D
TS12: surface ectoderm	Present	Regionally restricted	6D	Expression was detected in ventral surface ectodermal cells.
TS12: rhombomere floor plate	Weak		6D

Structure	Level	Pattern	Image	Note
TS12: node	Present		6E
TS12: embryo	Present	Regionally restricted	6E	Expressing cells lie immediately caudal to the developing somites. No expression is observed in tissues located rostral to the node, such as the floor plate or notochord.
TS12: neural plate	Absent		6E
TS12: notochord	Absent		6E

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		6F
TS13: surface ectoderm	Present	Regionally restricted	6F	Expression was detected in the surface ectoderm adjacent to rhombomere 4 and 5.
TS13: head mesenchyme	Present	Regionally restricted	6F	At the 8 somite stage, expression is becoming downregulated in the mesenchymal cells adjacent to rhombomere 4.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Absent		7A
TS13: head	Present	Regionally restricted	7A	Expression was detected in cells adjacent and caudal to the developing otic vesicle. Expression was no longer observed in the mesenchymal cells underlying rhombomere 4.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 4	Absent		7C
TS13: head	Present	Regionally restricted	7C	Expression was detected in cells adjacent and caudal to the developing otic vesicle. Expression was no longer observed in the mesenchymal cells underlying rhombomere 4.

Structure	Level	Pattern	Image	Note
TS13: 2nd branchial pouch	Present	Regionally restricted	7D	Expression was detected in surface ectodermal cells.
TS13: 2nd branchial arch mesenchyme	Absent		7D
TS13: lateral plate mesoderm	Present		7D	At the level of rhombomeres 5 and 6.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		7E	At the level of rhombomeres 5 and 6.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Present		7F	At the level of rhombomeres 5 and 6.

Structure	Level	Pattern	Image	Note
TS14: 3rd branchial arch	Present		8A
TS14: caudal neuropore	Present	Regionally restricted	8A	Expression is upregulated in the dorsal region.

Structure	Level	Pattern	Image	Note
TS16: future hindbrain	Absent		8C

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Present	Regionally restricted	8D	Expression was detected in lateral mesenchymal cells adjacent to rhombomere 6 and which appear to form a stream of cells extending ventrally toward the developing third branchial arch.
TS14: 2nd branchial pouch	Present	Regionally restricted	8D	Expression was detected in surface ectodermal cells of the second branchial pouch.
TS14: rhombomere 6	Absent		8D

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Present	Regionally restricted	8E	Expression was in lateral mesenchymal cells adjacent to rhombomere 6 and which appear to form a stream of cells extending ventrally toward the developing third branchial arch. Only cells immediately adjacent to the surface ectoderm showed expression.
TS14: surface ectoderm	Present	Regionally restricted	8E	Expression was detected in surface ectodermal cells overlying the third branchial arch.

Structure	Level	Pattern	Image	Note
TS14: embryo mesenchyme	Present	Regionally restricted	8F	Expression was in lateral mesenchymal cells adjacent to rhombomere 6 and which appear to form a stream of cells extending ventrally toward the developing third branchial arch. Only cells immediately adjacent to the surface ectoderm showed expression.
TS14: surface ectoderm	Present	Regionally restricted	8F	Expression was detected in surface ectodermal cells overlying the third branchial arch.

Structure	Level	Pattern	Image	Note
TS16: neural tube roof plate	Present