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Gene Expression Data
Assay Details
Assay
Reference: J:26525 Stoetzel C, et al., Dorso-ventral and rostro-caudal sequential expression of M-twist in the postimplantation murine embryo. Mech Dev. 1995 Jun;51(2-3):251-63
Assay type: RNA in situ
MGI Accession ID: MGI:5439806
Gene symbol: Twist1
Gene name: twist basic helix-loop-helix transcription factor 1
Probe: SC7
Probe preparation: Antisense labelled with digoxigenin RNA
Assay notes: Some of the ages are assigned by curator based on morphological criteria supplied by the authors.
Results
Specimen 2A: embryonic day 7.0 (more )
Note: Early primitive streak stage.
Structure Level Pattern Image Note
TS10: amniotic fold Present 2A
TS10: extraembryonic ectoderm Present 2A
TS10: extraembryonic mesoderm Present 2A
TS10: allantois Present Regionally restricted 2A Expression is in the anterior part of the allantois.
TS10: primitive streak Weak 2A
TS10: embryo mesoderm Weak Regionally restricted 2A Expression is in the mesoderm adjacent to the primitive streak.
TS10: amniotic cavity Present Single cells 2A

Specimen 2B: embryonic day 7.0 (more )
Note: Early primitive streak stage.
Structure Level Pattern Image Note
TS10: primitive streak Weak 2B
TS10: extraembryonic component Present 2B

Specimen 4A: embryonic day 7.5 (more )
Note: Head process stage.
Structure Level Pattern Image Note
TS11: extraembryonic mesoderm Present 4A
TS11: primitive streak Present 4A
TS11: allantois Present 4A
TS11: amnion Present 4A
TS11: amniotic cavity Present Scattered 4A
TS11: ectoplacental cone Absent 4A

Specimen 4B1: embryonic day 7.5 (more )
Note: Head process stage.
Structure Level Pattern Image Note
TS11: primitive streak Present 4B1
TS11: amniotic cavity Present Scattered 4B1

Specimen 4B2: embryonic day 7.5 (more )
Note: Head process stage.
Structure Level Pattern Image Note
TS11: primitive streak Present 4B2
TS11: amniotic cavity Present Scattered 4B2

Specimen 4B3: embryonic day 7.5 (more )
Note: Head process stage.
Structure Level Pattern Image Note
TS11: primitive streak Present 4B3
TS11: amniotic cavity Present Scattered 4B3

Specimen 4B4: embryonic day 7.5 (more )
Note: Head process stage.
Structure Level Pattern Image Note
TS11: primitive streak Present 4B4
TS11: amniotic cavity Present Scattered 4B4

Specimen 4B5: embryonic day 7.5 (more )
Note: Head process stage.
Structure Level Pattern Image Note
TS11: primitive streak Present 4B5
TS11: amniotic cavity Present Scattered 4B5

Specimen 4B6: embryonic day 7.5 (more )
Note: Head process stage.
Structure Level Pattern Image Note
TS11: primitive streak Present 4B6
TS11: amniotic cavity Present Scattered 4B6

Specimen 7: embryonic day 8.0 (more )
Note: Early headfold stage. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS12: head mesenchyme Strong 7
TS12: allantois Present Regionally restricted 7 Expression is in the anterior part.
TS12: extraembryonic mesenchyme Present 7
TS12: primitive streak Weak 7
TS12: embryo mesenchyme Present Regionally restricted 7 Expression is in mesoderm cells beneath the headfold.
TS12: primitive endoderm Absent 7

Specimen 8A: embryonic day 8.5 (more )
Note: 8 somites. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: 1st branchial arch Very strong 8A
TS13: lateral plate mesoderm Present 8A
TS13: trunk paraxial mesenchyme Present 8A
TS13: head mesenchyme Strong Regionally restricted 8A Expression is strongest around the optic vesicles and is absent in the dorsal part.
TS13: trunk somite Present Regionally restricted 8A In the mid-lordotic region of the embryo.
TS13: allantois Weak 8A
TS13: trunk unsegmented mesenchyme Weak 8A
TS13: heart Absent 8A

Specimen 8B: embryonic day 8.5 (more )
Note: 8 somites. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: head mesenchyme Present Regionally restricted 8B Expression is ventral and around the optic vesicles.
TS13: 1st branchial arch mesenchyme Present 8B
TS13: lateral plate mesoderm Present 8B
TS13: trunk somite Present 8B

Specimen 8C: embryonic day 8.5 (more )
Note: 8 somites. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: head mesenchyme Present Regionally restricted 8C Expression is ventral and around the optic vesicles.
TS13: 1st branchial arch mesenchyme Present 8C
TS13: lateral plate mesoderm Present 8C
TS13: trunk somite Present 8C

Specimen 8D: embryonic day 8.5 (more )
Note: 8 somites. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: head mesenchyme Present Regionally restricted 8D Expression is around the otic placode.

Specimen 8E: embryonic day 8.5 (more )
Note: 8 somites. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: head mesenchyme Present Regionally restricted 8E Expression is around the optic vesicles.
TS13: 1st branchial arch mesenchyme Present 8E
TS13: branchial arch ectoderm Absent 8E
TS13: surface ectoderm Absent 8E Absent in head surface ectoderm.

Specimen 9A,A': embryonic day 8.5 (more )
Note: 12-14 somites, turning embryo. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: head mesenchyme Present Regionally restricted 9A , 9A' Expression is around the optic vesicles.
TS13: 1st branchial arch mesenchyme Present 9A , 9A'
TS13: 2nd branchial arch Present 9A , 9A'
TS13: lateral plate mesoderm Present 9A , 9A'
TS13: trunk somite Present 9A , 9A'
TS13: umbilical vein extraembryonic part Present 9A , 9A'
TS13: allantois Present Regionally restricted 9A' , 9A Expression is at the base.
TS13: heart Absent 9A' , 9A
TS13: trunk unsegmented mesenchyme Absent 9A' , 9A

Specimen 9B: embryonic day 8.5 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: head mesenchyme Present 9B
TS13: neural ectoderm Absent 9B
TS13: otic placode Absent 9B

Specimen 9C: embryonic day 8.5 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: trunk somite Strong 9C
TS13: neural tube Absent 9C

Specimen 9D: embryonic day 8.5 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS13: somite Present Regionally restricted 9D Expression is mainly dorsal and is more faintly as a ring around the middle part.

Specimen 9E: embryonic day 9.0 (more )
Note: 12 somites. The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: 1st branchial arch Present 9E
TS14: head mesenchyme Present 9E
TS14: allantois Present Regionally restricted 9E Expression is at the base.
TS14: heart Absent 9E
TS14: trunk unsegmented mesenchyme Absent 9E

Specimen 10A,A': embryonic day 9.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: forelimb bud Present 10A' , 10A
TS14: head mesenchyme Present 10A' , 10A
TS14: 1st branchial arch Strong 10A' , 10A
TS14: 2nd branchial arch Strong 10A' , 10A
TS14: somite Strong 10A' , 10A
TS14: allantois Strong Regionally restricted 10A' , 10A Expression is at the base.

Specimen 10B: embryonic day 9.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: 1st branchial arch mesenchyme Present 10B
TS14: head mesenchyme Present Regionally restricted 10B Around the optic vesicles.
TS14: surface ectoderm Absent 10B Absent in head surface ectoderm.
TS14: branchial arch ectoderm Absent 10B
TS14: optic vesicle Absent 10B

Specimen 10C: embryonic day 9.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: allantois Strong Regionally restricted 10C Expression is at the base.
TS14: trunk unsegmented mesenchyme Present 10C Caudal unsegmented mesenchyme of the tail.
TS14: tail bud Present Single cells 10C Expression is in some cells of the unclosed neural tube.

Specimen 10D: embryonic day 9.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: mesenchyme derived from lateral plate Strong 10D
TS14: somite Strong 10D
TS14: neural tube Absent 10D
TS14: notochord Absent 10D
TS14: surface ectoderm Absent 10D

Specimen 10E: embryonic day 9.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: mesenchyme derived from lateral plate Strong 10E
TS14: somite Strong 10E
TS14: pharyngeal region Weak 10E
TS14: neural tube Absent 10E
TS14: notochord Absent 10E
TS14: surface ectoderm Absent 10E

Specimen 10F: embryonic day 9.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: mesenchyme derived from lateral plate Strong 10F
TS14: somite Strong 10F
TS14: neural tube Absent 10F
TS14: notochord Absent 10F
TS14: surface ectoderm Absent 10F

Specimen 10G: embryonic day 9.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS14: somite Present Uniform 10G

Specimen 11B: embryonic day 10.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS16: 3rd branchial arch Present 11B
TS16: 4th branchial arch Present 11B
TS16: 1st branchial arch Weak 11B
TS16: 2nd branchial arch Weak 11B

Specimen 11C: embryonic day 10.0 (more )
Note: The authors did not specify which probe was used for this specimen, it is possible that a shorter probe was used: MGI:1276127.
Structure Level Pattern Image Note
TS16: hindlimb bud Present 11C


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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory