Gene Expression Data
Assay Details
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Assay
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Reference:
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J:66531
Kraus P, et al., Some distal limb structures develop in mice lacking Sonic hedgehog signaling. Mech Dev. 2001 Jan;100(1):45-58
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5439631
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
6A:
(close  )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
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Mutant Allele(s):
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En1tm2Alj/En1+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: limb ectoderm |
Present |
Regionally restricted |
6A
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Expression was detected in the ventral ectoderm.
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Specimen
6B:
embryonic day 11.0; En1tm2Alj/En1 +
(more  )
Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: limb ectoderm |
Present |
Regionally restricted |
6B
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Expression was detected in the ventral ectoderm.
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| TS18: apical ectodermal ridge |
Present |
Regionally restricted |
6B
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Expression was detected in the ventral apical ectodermal ridge.
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Specimen
6C:
(close )
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Genetic Background:
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either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
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Mutant Allele(s):
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En1tm2Alj/En1+
Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb ectoderm |
Present |
Regionally restricted |
6C
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Expression was detected in the ventral ectoderm. The overall anterior-posterior length of the expression domain did not fade anteriorly at E12.0 (in contrast to Fgf8 - see assay MGI:5439574).
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Specimen
6D:
embryonic day 12.0; En1tm2Alj/En1 +
(more )
Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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En1tm2Alj/En1+
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|
Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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|
Fixation:
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4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: limb ectoderm |
Present |
Regionally restricted |
6D
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Expression was detected in the ventral ectoderm.
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| TS19: apical ectodermal ridge |
Present |
Regionally restricted |
6D
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Expression was detected in the ventral apical ectodermal ridge.
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Analysis Tools
