Probe:	Gli1 probe12
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS18: embryo	Absent

Structure	Level	Pattern	Image	Note
TS19: embryo	Absent

Structure	Level	Pattern	Image	Note
TS20: hand pre-cartilage condensation	Present	Regionally restricted	4A	Two domains of expression were detected in the forelimb, likely delineating the cartilaginous condensations of the zeugopod and autopod.
TS20: radius-ulna pre-cartilage condensation	Present	Regionally restricted	4A	Two domains of expression were detected in the forelimb, likely delineating the cartilaginous condensations of the zeugopod and autopod.

Structure	Level	Pattern	Image	Note
TS20: foot pre-cartilage condensation	Present	Regionally restricted	4B	Two domains of expression were detected in the hindlimb, likely delineating the cartilaginous condensations of the zeugopod and autopod.
TS20: hindlimb pre-cartilage condensation	Present	Regionally restricted	4B	Two domains of expression were detected in the hindlimb, likely delineating the cartilaginous condensations of the zeugopod and autopod.

Structure	Level	Pattern	Image	Note
TS21: forelimb	Present	Regionally restricted	4C	Two linear stripes of expression were detected in the forelimb suggestive of the metacarpals.

Structure	Level	Pattern	Image	Note
TS21: hindlimb	Present	Regionally restricted	4D	Two linear stripes of expression were detected in the hindlimb.
TS21: hindlimb digit pre-cartilage condensation	Present	Regionally restricted	4D	Two rings of expression were detected in the hindlimb, likely representing the presence of two phalanges.

Structure	Level	Pattern	Image	Note
TS20: hand pre-cartilage condensation	Present		4E

Structure	Level	Pattern	Image	Note
TS20: foot pre-cartilage condensation	Present		4F

Structure	Level	Pattern	Image	Note
TS21: metacarpus pre-cartilage condensation	Present	Regionally restricted	4G	Expression was detected in 2 stripes for each region of the forming metacarpals.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit 2 phalanx pre-cartilage condensation	Present	Regionally restricted	4H	Two rings of expression delineated the proximal two phalanges of digits 2-5.
TS21: hindlimb digit 3 phalanx pre-cartilage condensation	Present	Regionally restricted	4H	Two rings of expression delineated the proximal two phalanges of digits 2-5.
TS21: hindlimb digit 4 phalanx pre-cartilage condensation	Present	Regionally restricted	4H	Two rings of expression delineated the proximal two phalanges of digits 2-5.
TS21: hindlimb digit 5 phalanx pre-cartilage condensation	Present	Regionally restricted	4H	Two rings of expression delineated the proximal two phalanges of digits 2-5.
TS21: metatarsus pre-cartilage condensation	Present	Regionally restricted	4H	Expression was detected in 2 stripes for each region of the forming metatarsals.