Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:66531
Kraus P, et al., Some distal limb structures develop in mice lacking Sonic hedgehog signaling. Mech Dev. 2001 Jan;100(1):45-58
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5439574
|
|
Gene symbol:
|
Fgf8
|
|
Gene name:
|
fibroblast growth factor 8
|
|
Probe:
|
Fgf8 probe46
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
3A:
(close  )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3B:
embryonic day 10.5
(more  )
Specimen
3B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
not shown:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 10.75
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud apical ectodermal ridge |
Present |
Regionally restricted |
|
By E10.75 expression was restricted to a small region of the posterior forelimb apical ectodermal ridge in the mutant.
|
Specimen
3C:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3D:
embryonic day 11.5
(more )
Specimen
3D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
3E:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ * C57BL/6J) or (involves: 129S1/Sv * 129X1/SvJ * Swiss Webster)
|
|
Mutant Allele(s):
|
Shhtm1Chg/Shhtm1Chg
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hand |
Present |
Regionally restricted |
3E
|
Expression was severely restricted in the anterior-posterior expanse, remaining strongest only in a small posterior region.
|
Specimen
3F:
embryonic day 12.5
(more )
Specimen
3F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Analysis Tools