Probe:	Bapx1 probe1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS12: somite	Present	Regionally restricted	8A/B	Expression was detected in the presclerotome region of the somite.
TS12: lateral plate mesoderm	Present	Regionally restricted	8A/B	Expression was detected in lateral plate mesoderm overlying the future gut endoderm.

Structure	Level	Pattern	Image	Note
TS13: lateral plate mesoderm	Strong	Regionally restricted	8C/D	Expression was detected in lateral plate mesoderm surrounding the midgut epithelium.
TS13: somite	Present	Regionally restricted	8C/D	Expression was detected in the sclerotome.

Structure	Level	Pattern	Image	Note
TS14: mesenchyme derived from lateral plate	Strong	Regionally restricted	8E/F	Expression was detected in lateral plate mesoderm surrounding the midgut epithelium.
TS14: sclerotome	Strong		8E/F

Structure	Level	Pattern	Image	Note
TS14: gut mesenchyme	Strong		8G/H
TS14: sclerotome	Strong		8G/H

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		8I/J

Structure	Level	Pattern	Image	Note
TS15: 1st branchial arch	Present	Regionally restricted	8K/L	Highest intensity was in the inferior portion on the first branchial arch.

Structure	Level	Pattern	Image	Note
TS17: gut mesentery	Strong		9A/B
TS17: sclerotome	Strong		9A/B

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Strong		9C/D
TS17: gut mesentery	Strong		9C/D
TS17: sclerotome	Strong		9C/D

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Strong		9E/F

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Strong		9G/H

Structure	Level	Pattern	Image	Note
TS17: sclerotome	Strong		9I/J
TS17: hindgut mesentery	Strong	Regionally restricted	9I/J	Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
TS17: ventral mesogastrium	Strong	Regionally restricted	9I/J	Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.

Structure	Level	Pattern	Image	Note
TS20: Meckel's cartilage	Present		9K/L
TS20: vertebral cartilage condensation	Present		9K/L
TS20: limb pre-cartilage condensation	Present	Regionally restricted	9K/L	Expression was detected in the cartilaginous condensations.
TS20: axial skeleton	Present	Regionally restricted	9K/L	Expression was detected in both the vertebral body and the intervertebral disc.

Structure	Level	Pattern	Image	Note
TS20: hindgut mesentery	Present	Regionally restricted	10A/B	Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
TS20: ventral mesogastrium	Present	Regionally restricted	10A/B	Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
TS20: axial skeleton	Strong	Regionally restricted	10A/B	Strong expression was detected in the prevertebrae. Expression was detected in both the vertebral body and the intervertebral disc.
TS20: hindlimb pre-cartilage condensation	Strong		10A/B

Structure	Level	Pattern	Image	Note
TS20: hindgut mesentery	Present	Regionally restricted	10C/D	Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
TS20: ventral mesogastrium	Present	Regionally restricted	10C/D	Expression was detected in the lesser omentum (ventral mesoderm of the stomach) and in discreet patches surrounding the hindgut.
TS20: Meckel's cartilage	Present		10C/D
TS20: axial skeleton	Strong	Regionally restricted	10C/D	Strong expression was detected in the prevertebrae. Expression was detected in both the vertebral body and the intervertebral disc.
TS20: hindlimb pre-cartilage condensation	Strong		10C/D

Structure	Level	Pattern	Image	Note
TS22: gut mesentery	Present		10E/F
TS22: embryo	Present	Regionally restricted	10E/F	Expression was detected qualitatively in all the same structures as earlier stages.

Structure	Level	Pattern	Image	Note
TS22: gut mesentery	Present		10G/H
TS22: embryo	Present	Regionally restricted	10G/H	Expression was detected qualitatively in all the same structures as earlier stages.

Structure	Level	Pattern	Image	Note
TS24: embryo	Present	Regionally restricted	10I/J	Expression was detected qualitatively in all the same structures as earlier stages, but by E16.5 there is a significant decrease in the level of expression.
TS24: vertebra	Present	Regionally restricted	10I/J	Expression was strongest in the most caudal prevertebrae and has decreased significantly in more rostral prevertebrae.

Structure	Level	Pattern	Image	Note
TS24: limb skeleton	Present	Regionally restricted	10K/L	Expression was strongest in the most distal cartilaginous condensations, and decreases in a distoproximal gradient.
TS24: limb digit	Strong		10K/L

Structure	Level	Pattern	Image	Note
TS16: branchial arch	Present		11A/B
TS16: trunk sclerotome	Present		11A/B

Structure	Level	Pattern	Image	Note
TS20: tail mesenchyme	Present	Regionally restricted	11E/F	Expression was detected in the mesoderm surrounding the notochord. Expression was highest in cells closest to the notochord and decreased with increasing distance from the notochord.

Structure	Level	Pattern	Image	Note
TS24: otic cartilage	Present		11J/K

Structure	Level	Pattern	Image	Note
TS20: axial skeleton	Present	Regionally restricted	12A/B	Expression was detected in the prevertebrae of the entire axial skeleton.
TS20: somite	Present	Regionally restricted	12A/B	Expression was more densely localized in the caudal half of the somite.

Structure	Level	Pattern	Image	Note
TS21: limb cartilage condensation	Present		12F/G