Gene Expression Data
Assay Details
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Assay
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Reference:
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J:39658
Buscher D, et al., Evidence for genetic control of Sonic hedgehog by Gli3 in mouse limb development. Mech Dev. 1997 Mar;62(2):175-82
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5437463
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
A upper left:
embryonic day 10.0
(more  )
Specimen
A upper left:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
A upper middle:
embryonic day 11.0
(more )
Specimen
A upper middle:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
A upper right:
embryonic day 12.0
(more )
Specimen
A upper right:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
2A upper right
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Expression was restricted to the posterior side.
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Specimen
A lower left:
embryonic day 10.0
(more )
Specimen
A lower left:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
A lower middle:
embryonic day 11.0
(more )
Specimen
A lower middle:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
A lower right:
embryonic day 12.0
(more )
Specimen
A lower right:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
2A lower right
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Expression was restricted to the posterior side.
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Specimen
B upper left:
embryonic day 10.0; Gli3Xt/ Gli3Xt
(more )
Specimen
B upper left:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
B upper middle:
embryonic day 11.0; Gli3Xt/ Gli3Xt
(more )
Specimen
B upper middle:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
Regionally restricted |
2B upper middle
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Expression was restricted to the posterior side and an additional anterior domain. This anterior domain was much smaller in size than the posterior domain.
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Specimen
B upper right:
embryonic day 12.0; Gli3Xt/ Gli3Xt
(more )
Specimen
B upper right:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 12.0
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: forelimb |
Present |
Regionally restricted |
2B upper right
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Expression was restricted to the posterior side and an additional anterior domain. This anterior domain was much smaller in size than the posterior domain.
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Specimen
B lower left:
embryonic day 10.0; Gli3Xt/ Gli3Xt
(more )
Specimen
B lower left:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
B lower middle:
embryonic day 11.0; Gli3Xt/ Gli3Xt
(more )
Specimen
B lower middle:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Specimen
B lower right:
embryonic day 12.0-12.5; Gli3Xt/ Gli3Xt
(more )
Specimen
B lower right:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 12.0-12.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindlimb |
Present |
Regionally restricted |
2B lower right
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Expression was restricted to the posterior side and an additional anterior domain. This anterior domain was much smaller in size than the posterior domain.
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Specimen
not shown:
embryonic day 9.5; Gli3Xt/ Gli3Xt
(more )
Specimen
not shown:
(close )
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Genetic Background:
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involves: 101/H * C3H/HeH * CBA/H
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Mutant Allele(s):
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Gli3Xt/Gli3Xt
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: limb |
Present |
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Expression is the same as in the wild-type control.
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Specimen
not shown:
embryonic day 9.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: limb |
Present |
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Analysis Tools