Gene Expression Data
Assay Details
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Assay
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Reference:
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J:185683
Wu B, et al., Nfatc1 coordinates valve endocardial cell lineage development required for heart valve formation. Circ Res. 2011 Jul 8;109(2):183-92
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5436656
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Gene symbol:
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Casp3
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Gene name:
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caspase 3
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Antibody:
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anti-cleaved Caspase-3
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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The antibody used recognizes the cleaved form of the protein.
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Results
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Specimen
S3C:
embryonic day 13.5
(more  )
Specimen
S3C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pulmonary valve |
Weak |
Single cells |
S3C
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Expression is in isolated cells at the edge of the primitive pulmonary valve leaflets.
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Specimen
S3D:
(close )
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Genetic Background:
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B6.129S2-Nfatc1tm1Glm
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Mutant Allele(s):
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Nfatc1tm1Glm/Nfatc1tm1Glm
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pulmonary valve |
Strong |
Regionally restricted |
S3D
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Expression is at the edge of the primitive pulmonary valve leaflets.
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Specimen
Not shown E10.5 +/+:
embryonic day 10.5
(more )
Specimen
Not shown E10.5 +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown E10.5 -/-:
(close )
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Genetic Background:
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B6.129S2-Nfatc1tm1Glm
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Mutant Allele(s):
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Nfatc1tm1Glm/Nfatc1tm1Glm
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown E11.5 +/+:
embryonic day 11.5
(more )
Specimen
Not shown E11.5 +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown E11.5 -/-:
(close )
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Genetic Background:
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B6.129S2-Nfatc1tm1Glm
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Mutant Allele(s):
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Nfatc1tm1Glm/Nfatc1tm1Glm
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Analysis Tools