Gene Expression Data
Assay Details
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Assay
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Reference:
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J:49210
Bell SM, et al., The loss of ventral ectoderm identity correlates with the inability to form an AER in the legless hindlimb bud. Mech Dev. 1998 Jun;74(1-2):41-50
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5436578
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Gene symbol:
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Cd44
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Gene name:
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CD44 antigen
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Antibody:
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anti-CD44 (IM7)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1D:
embryonic day 9.5
(more  )
Note: 28-29 somites. Limb stage 0.5.
Specimen
1D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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28-29 somites. Limb stage 0.5.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: hindlimb ridge ectoderm |
Present |
Regionally restricted |
1D
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Expression was detected in ventral ectoderm across almost the entire ventral surface.
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Specimen
1I:
embryonic day 10.0
(more )
Note: 29-31 somites. Limb stage 1.
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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29-31 somites. Limb stage 1.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud ectoderm |
Present |
Regionally restricted |
1I
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Expression was detected in ventral ectoderm. The domain co-localizes with the cells of the thickening ventro-distal margin.
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Specimen
1N:
embryonic day 10.0
(more )
Note: 31-33 somites. Limb stage 1.5.
Specimen
1N:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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31-33 somites. Limb stage 1.5.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud ectoderm |
Present |
Regionally restricted |
1N
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Expression was detected in ventral ectoderm. The domain co-localizes with the cells of the thickening ventro-distal margin.
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Specimen
1R:
embryonic day 10.0-10.5
(more )
Note: 33-36 somites. Limb stage 2.
Specimen
1R:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0-10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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33-36 somites. Limb stage 2.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: hindlimb bud ectoderm |
Present |
Regionally restricted |
1R
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Expression was detected in ventral ectoderm. The domain co-localizes with the cells of the thickening ventro-distal margin.
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