Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:65646
Tomotsune D, et al., Regulation of hoxb3 expression in the hindbrain and pharyngeal arches by rae28, a member of the mammalian polycomb group of genes. Mech Dev. 2000 Nov;98(1-2):165-9
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5435228
|
|
Gene symbol:
|
Hoxb3
|
|
Gene name:
|
homeobox B3
|
|
Probe:
|
Hox-2.7 probe2
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
2A:
embryonic day 8.0
(more  )
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
2A
|
The anterior boundary of expression corresponds to the rhombomere 4/5 boundary.
|
Specimen
2E:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Phc1tm1Os/Phc1tm1Os
|
|
Age:
|
embryonic day 8.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: rhombomere |
Present |
Regionally restricted |
2E
|
The anterior boundary of expression corresponds to the rhombomere 4/5 boundary as in wild type.
|
Specimen
2B:
embryonic day 9.5
(more )
Specimen
2B:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 4 |
Absent |
|
2B
|
The anterior boundary of expression corresponds to the rhombomere 4/5 boundary.
|
| TS15: rhombomere 5 |
Present |
|
2B
|
The anterior boundary of expression corresponds to the rhombomere 4/5 boundary.
|
Specimen
2C:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Phc1tm1Os/Phc1tm1Os
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: rhombomere 4 |
Absent |
|
2C
|
The anterior boundary of expression corresponds to the rhombomere 4/5 boundary as in wild type.
|
| TS15: rhombomere 5 |
Present |
|
2C
|
The anterior boundary of expression corresponds to the rhombomere 4/5 boundary as in wild type.
|
Specimen
2F:
embryonic day 9.5
(more )
Specimen
2F:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
2G:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Phc1tm1Os/Phc1tm1Os
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
3A:
embryonic day 11.5
(more )
Specimen
3A:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Phc1tm1Os/Phc1tm1Os
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
3B
|
The anterior boundary of ectopic expression extended to rhombomere 3 in the mutant embryo.
|
Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Phc1tm1Os/Phc1tm1Os
|
|
Age:
|
embryonic day 10.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Present |
|
|
The expression pattern was affected in the mutant.
|
| TS17: rhombomere 4 |
Present |
|
|
Expression was ectopic in rhombomere 4 in the mutant.
|
Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Phc1tm1Os/Phc1tm1Os
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: embryo |
Present |
|
|
The expression pattern was affected in the mutant.
|
| TS20: pons |
Present |
|
|
The anterior boundary of ectopic expression had extended to the pons by E12.5.
|
Specimen
5C:
embryonic day 9.5
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Phc1tm1Os/Phc1tm1Os
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: migrating neural crest |
Present |
|
5D
|
Expression was ectopic in regions with migrating neural crest cells from rhombomere 4.
|
|
Analysis Tools