Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:45681
Suda Y, et al., Cooperation between Otx1 and Otx2 genes in developmental patterning of rostral brain. Mech Dev. 1997 Dec;69(1-2):125-41
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:5433379
|
|
Gene symbol:
|
Wnt1
|
|
Gene name:
|
wingless-type MMTV integration site family, member 1
|
|
Probe:
|
Wnt1 probe1
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
|
Results
|
Specimen
3c:
embryonic day 9.5
(more  )
Specimen
3c:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
3c
|
Expression was detected in a stripe at the isthmus and dorsally it is expressed from the isthmus to part of the dorsal thalamus (p2 diencephalon). A negative region was noted in the anterior diencephalon.
|
| TS15: future brain |
Present |
Regionally restricted |
3c
|
Expression was detected in a stripe at the isthmus and dorsally it is expressed from the isthmus to part of the dorsal thalamus (p2 diencephalon). A negative region was noted in the anterior diencephalon.
|
| TS15: midbrain-hindbrain junction |
Present |
Regionally restricted |
3c
|
Expression was detected in a stripe at the isthmus and dorsally it is expressed from the isthmus to part of the dorsal thalamus (p2 diencephalon). A negative region was noted in the anterior diencephalon.
|
Specimen
3d:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 9.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Present |
Regionally restricted |
3d
|
Expression was more anteriorly located in the double heterozygote, but the stripe of expression was nearly unchanged. The negative region in the roof of the anterior diencephalon contained a line of expressing cells.
|
| TS15: future brain |
Present |
Regionally restricted |
3d
|
Expression was more anteriorly located in the double heterozygote, but the stripe of expression was nearly unchanged. The negative region in the roof of the anterior diencephalon contained a line of expressing cells.
|
| TS15: midbrain-hindbrain junction |
Present |
Regionally restricted |
3d
|
Expression was more anteriorly located in the double heterozygote, but the stripe of expression was nearly unchanged. The negative region in the roof of the anterior diencephalon contained a line of expressing cells.
|
Specimen
7a:
embryonic day 8.0
(more )
Note: 3 somite stage.
Specimen
7a:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3 somite stage.
|
Specimen
7b:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
3 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
|
7b
|
No difference in expression was found at the 3-somite stage.
|
Specimen
7c:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
7c:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
6 somite stage.
|
Specimen
7d:
(close )
|
Genetic Background:
|
involves: C57BL/6 * CBA
|
|
Mutant Allele(s):
|
Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
6 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
|
7d
|
The expression domain was greatly narrowed and anteriorly located at the 6-somite stage in the double heterozygotes.
|
|
Analysis Tools