Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45681
Suda Y, et al., Cooperation between Otx1 and Otx2 genes in developmental patterning of rostral brain. Mech Dev. 1997 Dec;69(1-2):125-41
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5433372
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Gene symbol:
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Gbx2
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Gene name:
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gastrulation brain homeobox 2
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Probe:
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Gbx2 probe8
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2c:
embryonic day 9.5
(more  )
Specimen
2c:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
2c
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Expression was detected in metencephalon adjacent to the isthmus anteriorly.
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Specimen
2d:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future hindbrain |
Present |
Regionally restricted |
2d
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The stripe of expression was shifted rostrally in the double heterozygote.
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Specimen
8i:
embryonic day 8.0
(more )
Note: 3 somite stage.
Specimen
8i:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
Regionally restricted |
8i
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The anterior end of normal expression was vague at the 3-somite stage.
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Specimen
8j:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somite stage.
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Specimen
8k:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
8k:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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Specimen
8l:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: midbrain-hindbrain junction |
Present |
Regionally restricted |
8l
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Expression was normal but shifted rostrally at the 6-somite stage in the double heterozygous mutant.
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Analysis Tools