Gene Expression Data
Assay Details
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Assay
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Reference:
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J:45681
Suda Y, et al., Cooperation between Otx1 and Otx2 genes in developmental patterning of rostral brain. Mech Dev. 1997 Dec;69(1-2):125-41
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5433305
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Gene symbol:
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Pax2
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Gene name:
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paired box 2
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Probe:
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Pax2 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
2a:
embryonic day 9.5
(more  )
Specimen
2a:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2b:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Regionally restricted |
2b
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Expression in the commissural plate was unchanged in the mutant.
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| TS15: midbrain-hindbrain junction |
Present |
Regionally restricted |
2b
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Expression in the isthmus shifted rostrally in the double heterozygotes. The characteristic stripe of expression was slightly enlarged.
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Specimen
8e:
embryonic day 8.0
(more )
Note: 3 somite stage. Also stained for Egr2 (Krox-20; assay MGI:5433375).
Specimen
8e:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somite stage. Also stained for Egr2 (Krox-20; assay MGI:5433375).
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Specimen
8f:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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3 somite stage. Also stained for Egr2 (Krox-20; assay MGI:5433375).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
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8f
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Expression was normal at the 3-somite stage in the double mutant.
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Specimen
8g:
embryonic day 8.0
(more )
Note: 6 somite stage.
Specimen
8g:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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Specimen
8h:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Mutant Allele(s):
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Otx1tm1Sia/Otx1+
Otx2tm1Sia/Otx2+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural ectoderm |
Present |
Regionally restricted |
8h
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Expression was normally concentrated, although anteriorly shifted in the double mutant.
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Analysis Tools