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Gene Expression Data
Assay Details
Assay
Reference: J:185962 Chen D, et al., Osteoblast-specific transcription factor Osterix (Osx) and HIF-1alpha cooperatively regulate gene expression of vascular endothelial growth factor (VEGF). Biochem Biophys Res Commun. 2012 Jul 20;424(1):176-81
Assay type: RT-PCR
MGI Accession ID: MGI:5433145
Gene symbol: Hif1a
Gene name: hypoxia inducible factor 1, alpha subunit
Probe: Hif-1 alpha-pA, Hif-1 alpha-pB
Assay notes: Quantitative RT-PCR. Transcript levels were normalized to Gapdh. The level of RNA from Osx-null calvaria was normalized to a value of 1.
Results Image: 2A Hif-1 alpha
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
wild type E18.5 TS26: chondrocranium Present Not Specified; total RNA Not Specified Not Specified
Osx-null E18.5 TS26: chondrocranium Present (a) Not Specified; total RNA involves: 129S7/SvEvBrd * C57BL/6 Sp7tm1Crm/Sp7tm1CrmNot Specified
Notes:
(a) Expression level was unchanged in the mutant compared to wild type.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/18/2025
MGI 6.24
The Jackson Laboratory