Probe:	Hfh2 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS9: embryo	Present	Uniform

Structure	Level	Pattern	Image	Note
TS11: embryo	Present	Uniform

Structure	Level	Pattern	Image	Note
TS13: future hindbrain neural crest	Present	Regionally restricted	1A	Expression was restricted to the premigratory dorsal-lateral most cranial neural crest in the hindbrain and part of the midbrain.
TS13: future midbrain neural crest	Present	Regionally restricted	1A	Expression was restricted to the premigratory dorsal-lateral most cranial neural crest in the hindbrain and part of the midbrain.
TS13: rhombomere 3	Absent		1A
TS13: rhombomere 5	Absent		1A

Structure	Level	Pattern	Image	Note
TS13: future hindbrain neural crest	Present	Regionally restricted	1B	Expression was restricted to the premigratory dorsal-lateral most cranial neural crest in the hindbrain and part of the midbrain.
TS13: future midbrain neural crest	Present	Regionally restricted	1B	Expression was restricted to the premigratory dorsal-lateral most cranial neural crest in the hindbrain and part of the midbrain.
TS13: rhombomere 3	Absent		1B
TS13: rhombomere 5	Absent		1B
TS13: neural tube roof plate	Present	Regionally restricted	1B	Expression was detected in the roofplate of the neural tube caudal to the hindbrain, coincident with cells fated to become neural crest.

Structure	Level	Pattern	Image	Note
TS13: future hindbrain neural crest	Present	Regionally restricted	1C	Expression was restricted to the premigratory dorsal-lateral most cranial neural crest in the hindbrain and part of the midbrain.
TS13: future midbrain neural crest	Present	Regionally restricted	1C	Expression was restricted to the premigratory dorsal-lateral most cranial neural crest in the hindbrain and part of the midbrain.
TS13: rhombomere 3	Absent		1C
TS13: rhombomere 5	Absent		1C

Structure	Level	Pattern	Image	Note
TS13: future hindbrain neural fold	Present	Regionally restricted	1D	Expression was restricted to two regions at the most dorsal lateral edges of the open neural folds.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord neural fold	Present	Regionally restricted	1E	Expression was restricted to the edges of the neural folds.

Structure	Level	Pattern	Image	Note
TS13: future spinal cord	Present	Regionally restricted	1F	Expression is in the dorsalmost part of the spinal cord before and after neural tube closure.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1G	Expression at the mesencephalic head fold level reveals that expression is in the epithelial cells of the neuroepithelium underlying mesenchymal cells undergoing an epithelial to mesenchymal transition.

Structure	Level	Pattern	Image	Note
TS14: future hindbrain	Present	Regionally restricted		Expression persists in cranial neural crest cells in the hindbrain through E9.0.

Structure	Level	Pattern	Image	Note
TS15: cranial preganglion	Present		1H
TS15: lens placode	Present		1H

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	1I	Expression was detected in the lateral portion of the developing neural tube.
TS15: migrating neural crest	Present	Regionally restricted	1I	Expression was detected in migrating neural crest cells of the trunk.
TS15: peripheral nervous system	Present	Regionally restricted	1I	Expression was detected in the sympathetic ganglia of the gut.

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Absent		2B
TS13: rhombomere 5	Absent		2B
TS13: rhombomere 4	Present		2B

Structure	Level	Pattern	Image	Note
TS13: rhombomere 3	Absent		2D
TS13: rhombomere 5	Absent		2D
TS13: rhombomere 4	Present	Regionally restricted	2D	The same cells are not expressing both Foxd3 (Hfh2) and Hoxb1. Expression was restricted to the furthest dorsolateral cells in rhombomere 4 and are not found in the remainder of the neural epithelium in this rhombomere.
TS13: future midbrain	Present	Regionally restricted	2D	Expression was restricted to the furthest dorsolateral cells.
TS13: rhombomere 2	Present	Regionally restricted	2D	Expression was restricted to the furthest dorsolateral cells.