Tg(CAG-XBP1/Luc)23Tiw
Transgene Detail
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| Symbol: |
Tg(CAG-XBP1/Luc)23Tiw |
| Name: |
transgene insertion 23, Takao Iwawaki |
| MGI ID: |
MGI:5432350 |
| Synonyms: |
ERAI-LUC |
| Transgene: |
Tg(CAG-XBP1/Luc)23Tiw Location: unknown
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| Alliance: |
Tg(CAG-XBP1/Luc)23Tiw page
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: Nucleotides 410-633 of a human XBP1 cDNA including a Kozak sequence and FLAG-tag coding sequence was fused to the Luciferase reporter and then placed under the control of the CAG promoter construct in the pCAX expression vector (pCAX is a variant of pCAGG). The XBP1 fragment lacks the DNA binding domain but includes a 26-nt endoplasmic reticulum stress specific intron. Under normal conditions, the mRNA of the fusion gene is not spliced and translation terminates at a stop codon near the junction of XBP1 and luciferase. During ER stress, the 26 nt intron is spliced out and translation terminates at the stop codon of luciferase.
(J:153222)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:153222 Iwawaki T, et al., Function of IRE1 alpha in the placenta is essential for placental development and embryonic viability. Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16657-62 |
| All: |
3 reference(s) |
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Analysis Tools
