Tg(Wnt1-flpe/ERT2)9455Dym
Transgene Detail
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| Symbol: |
Tg(Wnt1-flpe/ERT2)9455Dym |
| Name: |
transgene insertion 9455, Susan Dymecki |
| MGI ID: |
MGI:5432117 |
| Synonyms: |
Wnt1::FlpeERT2 |
| Transgene: |
Tg(Wnt1-flpe/ERT2)9455Dym Location: unknown
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| Alliance: |
Tg(Wnt1-flpe/ERT2)9455Dym page
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| Strain of Origin: |
(C57BL/6 x SJL)F2 or FVB
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Wnt1-flpe/ERT2)9455Dym expression driven by
1 gene
Transgene expression driven by:
| Organism |
Driver Gene |
Note |
| Not Specified |
Wnt1 |
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Mutation details: The construct was constructed by inserting a cassette containing the flpe/ERT2 coding and polyA sequences into a Wnt1 expression-vector. 11 kb of Wnt1 genomic DNA containing critical 3' enhancer sequences were included after the FLPe/ERT2 cassette. Tandem insulator sequences flanked the complete construct. The purified transgene was injected into fertilized eggs to produce transgenic animals. 11 lines were obtained and FLPe/ERT2 mRNA expression recapitulated endogenous Wnt1 expression. Two lines showing the highest levels of FLPe/ERT2 mRNA were characterized with respect to recombinase activity. Data from line 9455 was shown in detail.
(J:97320)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Wnt1
(species not specified)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:97320 Hunter NL, et al., Ligand-activated Flpe for temporally regulated gene modifications. Genesis. 2005 Mar;41(3):99-109 |
| All: |
1 reference(s) |
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Analysis Tools
