Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(CAG-flpe/ERT2)1Dym
Name:	transgene insertion 1, Susan Dymecki
MGI ID:	MGI:5432116
Synonyms:	CAG::FlpeERT2
Transgene:	Tg(CAG-flpe/ERT2)1Dym  Location: unknown
Alliance:	Tg(CAG-flpe/ERT2)1Dym page

Strain of Origin:

(C57BL/6 x SJL)F2 or FVB

Transgene Type:    Transgenic (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Tg(CAG-flpe/ERT2)1Dym expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note chicken CAG   Mutation details: The construct was constructed by inserting a cassette containing the FLPe/ERT2 coding and polyA sequences (enhanced FLP fused to a triple mutant estrogen receptor) into a vector containing the CAG promoter (chicken beta-actin promoter coupled to CMV early enhancer sequence). Tandem insulator sequences that include the chicken beta globin hypersensitive sites flanked the entire construct. The purified transgene was injected into fertilized eggs to produce transgenic animals. Eight lines were obtained. After treatment with tamoxifen, all showed ability to recombine and activate the R26::FRAP reporter when crossed to mice carrying that allele. The line showing the greatest induction of alkaline phophatase activity in embryos was characterized in further detail. Cre activity was found to be low in most adult tissues and not effective in the spleen or skin (J:186310). (J:97320)

Activity:	Tissue activity of this recombinase allele
Driver:	CAG (chicken) Summary of all recombinase alleles driven by CAG.

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:97320 Hunter NL, et al., Ligand-activated Flpe for temporally regulated gene modifications. Genesis. 2005 Mar;41(3):99-109
All:	3 reference(s)