Tg(Ctsk-cre/ERT2)4Hlk
Transgene Detail
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| Symbol: |
Tg(Ctsk-cre/ERT2)4Hlk |
| Name: |
transgene insertion 4, Bernard Hoflack |
| MGI ID: |
MGI:5431091 |
| Synonyms: |
CtsKCreERT2 |
| Transgene: |
Tg(Ctsk-cre/ERT2)4Hlk Location: unknown
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| Alliance: |
Tg(Ctsk-cre/ERT2)4Hlk page
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| Transgene Type: |
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Transgenic (Inducible, Recombinase) |
| Inducer: |
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tamoxifen |
| Mutation: |
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Insertion
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Tg(Ctsk-cre/ERT2)4Hlk expression driven by
1 gene
Transgene expression driven by:
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Mutation details: The transgenic construct was generated by insertion of the mouse Ctsk sequence (-3429 to +52) into a cre/ERT2 expression plasmid. A purified 1.7 kb fragment of the original vector was microinjected into C57BL/6 (B6) or FVB fertilized oocytes. Seven B6 and 2 FVB founder mice were identified and crossed to C57BL/6 (FVB founders were crossed + 8 generations to B6) animals. Quantitative RT-PCR (QPCR) was performed on 8-10 week-old animals and showed cre/ERT2 mRNA expression was significant only in bone tissue. Founder #4 on the original B6 background and #284 on the FVB background were crossed to Rosa-lacz reporter animals. Beta-galactosidase staining was observed in long bones and calvaria of tamoxifen-treated 8-10 week old animals and in embryos from pregnant females treated with tamoxifen at E13.5. Line #4 was studied in greater detail.
(J:186154)
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Activity: |
 Tissue activity of this recombinase allele
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Driver:
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Ctsk
(mouse)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:186154 Sanchez-Fernandez MA, et al., Transgenic mice for a tamoxifen-induced, conditional expression of the Cre recombinase in osteoclasts. PLoS One. 2012;7(5):e37592 |
| All: |
1 reference(s) |
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Analysis Tools
