Gene Expression Data
Assay Details
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Assay
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Reference:
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J:56466
Brohmann H, et al., The role of Lbx1 in migration of muscle precursor cells. Development. 2000 Jan;127(2):437-45
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5425262
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Gene symbol:
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Lbx1
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Gene name:
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ladybird homeobox 1
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Reporter:
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lacZ
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Probe:
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lacZ probe
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Probe preparation:
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Antisense RNA
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Results
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Specimen
1C right:
embryonic day 9.75; Lbx1tm1Cbm/Lbx1 +
(more  )
Specimen
1C right:
(close  )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermomyotome |
Present |
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1C right
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The expression pattern closely reproduces the expression pattern of the wild-type gene.
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Specimen
2A:
embryonic day 9.75; Lbx1tm1Cbm/Lbx1 +
(more )
Specimen
2A:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermomyotome |
Present |
Regionally restricted |
2A
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Expression is present in hypaxial muscle precursors in the lateral dermomyotome of occipital and cervical somites.
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| TS15: trunk dermomyotome |
Present |
Regionally restricted |
2A
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Expression is present in hypaxial muscle precursors in the somites at limb level.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1tm1Cbm
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: dermomyotome |
Present |
Regionally restricted |
2B
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As in wild type, expression is present in hypaxial muscle precursors in the lateral dermomyotome of occipital and cervical somites.
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| TS15: trunk dermomyotome |
Present |
Regionally restricted |
2B
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As in wild type, expression is present in hypaxial muscle precursors in the somites at limb level.
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Specimen
2C:
embryonic day 9.75; Lbx1tm1Cbm/Lbx1 +
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: 1st branchial arch mesenchyme |
Present |
Regionally restricted |
2C
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Expression is present in hypaxial muscle precursors that form the hypoglossal stream. They migrate toward the floor of the first branchial arch (where the tongue anlage is located).
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| TS15: forelimb bud mesenchyme |
Present |
Regionally restricted |
2C
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Expression is present in hypaxial muscle precursors.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1tm1Cbm
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: 1st branchial arch mesenchyme |
Present |
Regionally restricted |
2D
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Expression is present in hypaxial muscle precursors that form the hypoglossal stream. They migrate towards the floor of the first branchial arch, but this stream does not extend as far in mutants as is does in wild type.
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| TS15: forelimb bud mesenchyme |
Absent |
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2D
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Specimen
2E:
embryonic day 9.75; Lbx1tm1Cbm/Lbx1 +
(more )
Specimen
2E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1tm1Cbm
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Age:
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embryonic day 9.75
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4E:
embryonic day 10.75; Lbx1tm1Cbm/Lbx1 +
(more )
Note: This section includes the anterior half of the forelimb.
Specimen
4E:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This section includes the anterior half of the forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
4E
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Expression is present in differentiating muscle cells in the dorsal and ventral forelimb.
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| TS17: trunk mesenchyme |
Present |
Regionally restricted |
4E
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There are some expressing cells in the lateral plate mesoderm.
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Specimen
4F:
embryonic day 10.75; Lbx1tm1Cbm/ Lbx1tm1Cbm
(more )
Note: This section includes the anterior half of the forelimb.
Specimen
4F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1tm1Cbm
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This section includes the anterior half of the forelimb.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: forelimb bud mesenchyme |
Present |
Regionally restricted |
4F
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Unlike in wild type, there are not abundant numbers of expressing cells in the dorsal limb. In the ventral forelimb the expressing cells are restricted to the proximal protion in the anterior half of the limb.
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| TS17: trunk mesenchyme |
Present |
Regionally restricted |
4F
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Expressing cells are present in lateral plate mesoderm. These cells are more numerous in mutants compared to wild type.
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Specimen
Not shown:
embryonic day 10.75; Lbx1tm1Cbm/Lbx1 +
(more )
Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1+
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 1st branchial arch mesenchyme |
Present |
Regionally restricted |
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The endpoint of the hypoglossal stream in the floor of the first arch (where the tongue anlage is located) contains expressing cells.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd
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Mutant Allele(s):
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Lbx1tm1Cbm/Lbx1tm1Cbm
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Age:
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embryonic day 10.75
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: 1st branchial arch mesenchyme |
Present |
Regionally restricted |
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The endpoint of the hypoglossal stream in the floor of the first arch (where the tongue anlage is located) contains expressing cells; these cells are reduced in number in mutant embryos compared to control embryos.
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Analysis Tools