Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Prism)1849Htz
Name:	transgene insertion 1849, Nathaniel Heintz
MGI ID:	MGI:5317685
Synonyms:	Prism JD1849, Snap25::mcherry, Mobp::Yfp-HA, Aldh1L1::Cerulean-Myc, Tg(Snap25-mcherry,Mobp-YFP,Aldh1l1-Cerulean)1849Htz
Transgene:	Tg(Prism)1849Htz  Location: unknown
Alliance:	Tg(Prism)1849Htz page

Strain of Origin:

FVB

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: Three mouse bacterial artificial chromosome (BAC) transgenic constructs were co-injected to generate this Prism 1849 strain. The BAC RP23-290A18, containing the entire Snap25 gene, was modified by inserting mCherry into the translation start site (ATG) of Snap25. The BAC RP23-172H7, containing the entire Mobp gene, was modified by inserting a HA tagged YFP into the translation start site (ATG) of Mobp. The BAC RP23-7M9, containing the entire Aldh1l1 gene, was modified by inserting a Myc tagged Cerulean (CFP) into the translation start site (ATG) of Aldh1l1. Founder line 1849 was subsequently established. FISH of cultured astrocytes shows all three transgenes integrated into a single locus on chromosome 11. (J:189629)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:189629 Dougherty JD, et al., Mouse transgenesis in a single locus with independent regulation for multiple fluorophores. PLoS One. 2012;7(7):e40511
All:	2 reference(s)