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Gene Expression Data
Assay Details
Assay
Reference: J:182232 Tanaka Y, et al., Early ontogenic origin of the hematopoietic stem cell lineage. Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4515-20
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:5318543
Gene symbol: Runx1
Gene name: runt related transcription factor 1
Reporter: lacZ
Direct Detection
Assay notes: Embryos were fixed in 0.2% glutaraldehyde and fixed post X-gal reaction with 4% paraformaldehyde.
Results
Specimen Runx1wt/LacZ--whole mount: embryonic day 9.5; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 4_Runx1wt/LacZ-top Expression is present in definitive hematopoietic cells spread throughout the vasculature.
Specimen Runx1SACRE/LacZ--whole mount: embryonic day 9.5; Runx1tm1Medv/Runx1tm2.1(cre/Esr1*)Ims (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 4_Runx1SACRE/LacZ-top Expressing cells form a ring at the location where the blood island primordia would have been in E7.5 embryos.
Specimen Runx1wt/LacZ--magnified view: embryonic day 9.5; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 4_Runx1wt/LacZ-middle Expression is present in definitive hematopoietic cells spread throughout the vasculature.
Specimen Runx1SACRE/LacZ--magnified view: embryonic day 9.5; Runx1tm1Medv/Runx1tm2.1(cre/Esr1*)Ims (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 4_Runx1SACRE/LacZ-middle Expressing cells form a ring at the location where the blood island primordia would have been in E7.5 embryos.
Specimen Runx1wt/LacZ--dissected: embryonic day 9.5; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 4_Runx1wt/LacZ-bottom Expression is present in definitive hematopoietic cells spread throughout the vasculature.
Specimen Runx1SACRE/LacZ--dissected: embryonic day 9.5; Runx1tm1Medv/Runx1tm2.1(cre/Esr1*)Ims (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Regionally restricted 4_Runx1SACRE/LacZ-bottom Expressing cells form a ring at the location where the blood island primordia would have been in E7.5 embryos.
Specimen S1a: embryonic day 7.5-8.0; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS11: yolk sac Present S1A left
Specimen Runx1wt/LacZ--upper: embryonic day 9.5; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Single cells S8A upper left Expression is present in hematopoietic precursor cells.
Specimen Runx1wt/LacZ--lower: embryonic day 9.5; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Single cells S8A lower left Expression is present in hematopoietic precursor cells.
Specimen Runx1SACRE/LacZ--upper: embryonic day 9.5; Runx1tm1Medv/Runx1tm2.1(cre/Esr1*)Ims (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Single cells S8A upper right The hematopoietic precursor cells differentiated into endothelial cells in the absence of the transcription factor.
Specimen Runx1SACRE/LacZ--lower: embryonic day 9.5; Runx1tm1Medv/Runx1tm2.1(cre/Esr1*)Ims (more )
Structure Level Pattern Image Note
TS15: yolk sac Present Single cells S8A lower right The hematopoietic precursor cells differentiated into endothelial cells in the absence of the transcription factor.
Specimen Runx1wt/LacZ--E9.5-10: embryonic day 9.5-10.0; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS15: blood Present Single cells S8B upper left Areas of expression include the peripheral blood.
Specimen Runx1SACRE/LacZ--E9.5-10: embryonic day 9.5-10.0; Runx1tm1Medv/Runx1tm2.1(cre/Esr1*)Ims (more )
Structure Level Pattern Image Note
TS15: blood Present Single cells S8B upper right Areas of expression include the peripheral blood.
Specimen Runx1wt/LacZ--E10.5-11: embryonic day 10.5-11.0; Runx1tm1Medv/Runx1+ (more )
Structure Level Pattern Image Note
TS17: blood Present Single cells S8B lower left Areas of expression include the peripheral blood.
Specimen Runx1SACRE/LacZ--E10.5-11: embryonic day 10.5-11.0; Runx1tm1Medv/Runx1tm2.1(cre/Esr1*)Ims (more )
Structure Level Pattern Image Note
TS17: blood Absent S8B lower right
TS17: embryo Present S8B lower right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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