Gene Expression Data
Assay Details
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Assay
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Reference:
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J:180887
Lange A, et al., Fltp(T2AiCre): A new knock-in mouse line for conditional gene targeting in distinct mono- and multiciliated tissues. Differentiation. 2012 Feb;83(2):S105-13
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5316996
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Gene symbol:
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Cfap126
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Gene name:
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cilia and flagella associated protein 126
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Reporter:
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other (see notes)
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Antibody:
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anti-T2A (EGR) 5E6
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Detection system:
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Secondary antibody coupled to DyLight 549
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Assay notes:
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The T2A viral protein, included in the allele construct, was used as the reporter in this assay.
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Results
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Specimen
1E:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Cfap126tm1.1(icre)Heli/Cfap126+
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Age:
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embryonic day 7.75
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: node |
Present |
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1E merge
,
1E TA2(5E6)
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Staining is present in embryonic node cilia in the nodal pit and crown.
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Specimen
3G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6J
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Mutant Allele(s):
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Cfap126tm1.1(icre)Heli/Cfap126+
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: organ of Corti |
Present |
Regionally restricted |
3G T2A (5E6)
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Expression is present in the outer hair cells, in this case row 2. It is detected in the kinocilia and surrounds the basal body that anchors the kinocilium to the plasma membrane.
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Analysis Tools
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