Tg(Nefh/EGFP)1Eyer
Transgene Detail
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| Symbol: |
Tg(Nefh/EGFP)1Eyer |
| Name: |
transgene insertion 1, Joel Eyer |
| MGI ID: |
MGI:5315129 |
| Synonyms: |
NFH-GFP, Tg(Nefh-GFP) |
| Transgene: |
Tg(Nefh/EGFP)1Eyer Location: unknown
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| Alliance: |
Tg(Nefh/EGFP)1Eyer page
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| Transgene Type: |
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Transgenic (Inserted expressed sequence) |
| Mutation: |
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Insertion
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Tg(Nefh/EGFP)1Eyer expresses
1 gene
Transgene expresses:
| Organism |
Expressed Gene |
Note |
| mouse |
Nefh (MGI:97309) |
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Mutation details: The transgene comprises ~15 kb of the mouse neurofilament, heavy polypeptide (Nefh) gene - extending from ~3 kb upstream of the translation initiation codon to the middle of exon 4 - joined in-frame to the EGFP coding sequence, which is followed by the SV40 polyadenylation signal and a neomycin resistance cassette. The encoded fusion protein lacks a major part of the NEFH carboxyl (C-) terminal domain, including only 45 lysine-serine-proline (KSP) repeats from the beginning of the C-terminal. EGFP signal is detected specifically in neurons throughout the brain, spinal cord and peripheral nervous system of transgenic mice, especially strongly in the optic nerve, medulla and sciatic nerve, and is transported down axons of motor neurons, colocalizing with alpha-bungarotoxin at neuromuscular junctions. No expression is observed in astrocytes or Schwann cells nor in any organ outside the nervous system that has been examined.
(J:182279)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 2 strains available
Cell Lines: 0 lines available
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| Original: |
J:182279 Letournel F, et al., Neurofilament high molecular weight-green fluorescent protein fusion is normally expressed in neurons and transported in axons: a neuronal marker to investigate the biology of neurofilaments. Neuroscience. 2006;137(1):103-11 |
| All: |
2 reference(s) |
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Analysis Tools
