Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Igs3tm1.1(ACTB-EGFP*)Luo
Name:	intergenic site 3; targeted mutation 1.1, Liqun Luo
MGI ID:	MGI:5314259
Synonyms:	Iis3tm1.1(ACTB-EGFP*)Luo, M10TG, Miya10TG, Tg(ACTB-EGFP*)10Luo
Gene:	Igs3  Location: unknown  Genetic Position: Chr10, Syntenic
Alliance:	Igs3tm1.1(ACTB-EGFP*)Luo page

Germline Transmission:	Earliest citation of germline transmission: J:181830
Parent Cell Line:	R1 (ES Cell)
Strain of Origin:	(129X1/SvJ x 129S1/Sv)F1-Kitl+

Allele Type:    Targeted (Null/knockout, Reporter) Mutation:    Insertion   Mutation details: The pMADMalpha transgene was designed with the CMV enhancer/chicken beta-actin core promoter (pCA) promoter, a frt site, the MADM GT cassette, and second frt site, the MADM TG cassette, a third frt site, and an SV40 T-antigen poly(A) signal. The MADM GT cassette used here has the N-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP first exon; nucleotides 1-274), a beta-globin intronic sequence (containing a loxP-flanked neomycin resistance gene), and ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus (tdT3MycATG-less). The MADM TG cassette used here has an ATG start codon, a beta-globin intronic sequence (containing a loxP-flanked neomycin resistance gene), and the C-terminal portion of a mutant enhanced green fluorescent protein (mut4EGFP second exon; nucleotides 275-735 including stop codon). The transgene was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. ES cells were screened for intact single-copy transgenes with integration in intergenic regions close to any centromere. ES cells were identified with an intact single-copy of the transgene inserted into an intergenic region at ~21 Mbp on chromosome 10 between the Ahi1 and Myb loci; this new insertion locus was named Miya10 (or M10). The transgene inserted into an intergenic region at ~21 Mbp on chromosome 10 between the Ahi1 and Myb loci; this new insertion locus was named Miya10 (or M10). ]Cre-mediated recombination removed the neo cassette. (J:181830)

Generation of the Igs3tm1.1(ACTB-EGFP*)Luo and Igs3tm2.1(ACTB-EGFP*,-tdTomato)Luo alleles

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Igs3 Mutation:	2 strains or lines available

Original:	J:181830 Tasic B, et al., Extensions of MADM (mosaic analysis with double markers) in mice. PLoS One. 2012;7(3):e33332
All:	1 reference(s)