Summary | Transgene origin | Transgene description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Tg(Krt8-cre/ERT2,-GFP)1Xin
Name:	transgene insertion 1, Li Xin
MGI ID:	MGI:5314229
Synonyms:	K8-CreERT2, Tg(Krt8-cre/ERT2)1Xin
Transgene:	Tg(Krt8-cre/ERT2,-GFP)1Xin  Location: unknown
Alliance:	Tg(Krt8-cre/ERT2,-GFP)1Xin page

Strain of Origin:

C57BL/6J

Transgene Type:    Transgenic (Inducible, Recombinase, Reporter) Inducer:    tamoxifen Mutation:    Insertion   Tg(Krt8-cre/ERT2,-GFP)1Xin expression driven by 1 gene Transgene expression driven by: Organism Driver Gene Note mouse Krt8 (MGI:96705)   Mutation details: A BAC clone (RP23-234D2) was modified by recombineering methods to insert the cre/ERT2 fusion sequence at the translational start codon of Krt8. An IRES-GFP expression cassette was inserted downstream of the cre sequence. The linearized BAC construct was microinjected into fertilized C57BL/6J oocytes. RT-PCR was used to screen founder mice and one line was chosen for characterization based on its cre/ERT2 expression pattern which closely recapitulated endogenous Krt8 expression. This line carries 2 copies of the transgene (based on quantitative PCR performed on genomic DNA) with a single insertion site. However, although GFP is detectable by RT-PCR and Western blot analysis, GFP fluorescence cannot be visualized by fluorescent microscopy in tissue sections or by FACS analysis of dissociated prostate cells. (J:181459, J:181829)

Activity:	Tissue activity of this recombinase allele
Driver:	Krt8 (mouse) Summary of all recombinase alleles driven by Krt8. Comparative matrix view of recombinase activities Alliance mouse Krt8 gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:181459 Choi N, et al., Adult Murine Prostate Basal and Luminal Cells Are Self-Sustained Lineages that Can Both Serve as Targets for Prostate Cancer Initiation. Cancer Cell. 2012 Feb 14;21(2):253-65
All:	10 reference(s)