Reporter:	lacZ
	Direct Detection
Assay notes:	Embryos were fixed in a mixture of 2% formaldehyde and 0.2% glutaraldehyde.

Structure	Level	Pattern	Image	Note
TS15: hindgut	Present		2A

Structure	Level	Pattern	Image	Note
TS19: heart	Present		2B
TS19: neural tube	Present		2B

Structure	Level	Pattern	Image	Note
TS19: heart	Present		2C

Structure	Level	Pattern	Image	Note
TS19: notochord	Present		2D
TS19: neural tube floor plate	Present	Regionally restricted	2D
TS19: neural tube	Present	Regionally restricted	2D	Expressed in the ventral part corresponding to the basal plates.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	2E	Expressed in the floor plate, the most ventral part of the raphe and in the gray matter.
TS22: medulla oblongata floor plate	Present	Regionally restricted	2E
TS22: medulla oblongata lateral wall	Present	Regionally restricted	2E	Expressed in the most ventral part of the raphe and in the gray matter.

Structure	Level	Pattern	Image	Note
TS25: cerebral cortex intermediate zone	Strong		2F
TS25: cortical plate	Present		2F
TS25: cortical subplate	Present		2F
TS25: hippocampus	Strong		2F

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex intermediate zone	Present	Widespread	2G	Expressed in the whole region from the intermediate to the marginal zone.
TS26: cerebral cortex marginal zone	Present	Widespread	2G	Expressed in the whole region from the intermediate to the marginal zone.
TS26: cortical plate	Present	Widespread	2G	Expressed in the whole region from the intermediate to the marginal zone.
TS26: hippocampus	Present		2G

Structure	Level	Pattern	Image	Note
TS26: testis	Present		2H

Structure	Level	Pattern	Image	Note
TS26: ovary	Present		2I

Structure	Level	Pattern	Image	Note
TS24: skin	Present		2J

Structure	Level	Pattern	Image	Note
TS26: oral epithelium	Present	Regionally restricted	2K	Expressed in the mucosa.
TS26: tongue epithelium	Present	Regionally restricted	2K	Expressed in the mucosa.

Structure	Level	Pattern	Image	Note
TS26: adenohypophysis	Present		2L
TS26: neurohypophysis	Present		2L

Structure	Level	Pattern	Image	Note
TS26: adrenal medulla	Present	Regionally restricted	2M

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Present		3A
TS22: heart atrium	Present		3A
TS22: heart ventricle	Present		3A
TS22: lung	Present		3A
TS22: main olfactory bulb	Present		3A
TS22: nasal cavity epithelium	Present		3A
TS22: urogenital sinus	Present		3A
TS22: pons	Present	Regionally restricted	3A	Expressed in the floor plate, the most ventral part of the raphe, and the gray matter.
TS22: medulla oblongata floor plate	Present	Regionally restricted	3A
TS22: medulla oblongata lateral wall	Present	Regionally restricted	3A	Expressed in the most ventral part of the raphe, and the gray matter.
TS22: spinal cord floor plate	Present	Regionally restricted	3A
TS22: spinal cord roof plate	Present	Regionally restricted	3A
TS22: spinal cord lateral wall	Present	Regionally restricted	3A	Expressed in the raphe region and the gray matter.
TS22: tongue muscle	Present	Regionally restricted	3A

Structure	Level	Pattern	Image	Note
TS24: heart atrium	Present		3B	Equal expression in the atrium as in the ventriculum.
TS24: heart ventricle	Present		3B	Equal expression in the atrium as in the ventriculum.
TS24: brown fat	Present		3B
TS24: cerebral cortex intermediate zone	Present		3B
TS24: dorsal root ganglion	Present		3B
TS24: lung	Present		3B
TS24: main olfactory bulb	Present		3B
TS24: medulla oblongata	Present		3B
TS24: pancreas	Present		3B
TS24: skeletal muscle	Present		3B
TS24: spinal cord	Present		3B
TS24: tongue	Present		3B
TS24: trigeminal ganglion	Present		3B
TS24: pituitary gland	Present		3B	Expression in both adenohypophysis and neurohypophysis.
TS24: tongue epithelium	Present	Regionally restricted	3B	Expressed in the mucosa covering the tongue.

Structure	Level	Pattern	Image	Note
TS24: brown fat	Present		3C Upper
TS24: heart atrium	Present		3C Upper
TS24: heart ventricle	Present		3C Upper
TS24: lung	Present		3C Upper
TS24: spinal cord roof plate	Absent		3C Upper
TS24: spinal cord floor plate	Present		3C Upper
TS24: spinal cord lateral wall	Present	Regionally restricted	3C Upper	Expressed in the raphe and gray matter.

Structure	Level	Pattern	Image	Note
TS24: dorsal root ganglion	Present		3C Lower
TS24: metanephros	Present		3C Lower
TS24: pancreas	Present		3C Lower
TS24: skeletal muscle	Present		3C Lower
TS24: spleen	Present		3C Lower
TS24: genital tubercle	Present		3C Lower	Expressed in the phalus.
TS24: spinal cord roof plate	Absent		3C Lower
TS24: spinal cord floor plate	Present		3C Lower
TS24: spinal cord lateral wall	Present	Regionally restricted	3C Lower	Expressed in the raphe and gray matter.

Structure	Level	Pattern	Image	Note
TS24: adrenal medulla	Present		4A
TS24: cerebral cortex	Present		4A
TS24: dorsal root ganglion	Present		4A
TS24: heart atrium	Present		4A
TS24: heart ventricle	Present		4A
TS24: lung	Present		4A
TS24: metanephros	Present		4A
TS24: pituitary gland	Present		4A
TS24: skeletal muscle	Present		4A
TS24: spinal cord	Present		4A
TS24: tongue	Present		4A

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Present	Regionally restricted	4C	Intense expression in the superficial intermediate zone.
TS24: cerebral cortex marginal layer	Weak		4C
TS24: cerebral cortex ventricular layer	Present		4C
TS24: cortical plate	Present		4C

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS20: notochord	Absent

Structure	Level	Pattern	Image	Note
TS22: primary sex cord	Present			Expressed in the future seminiferous tubules.
TS22: skin	Present
TS22: skeletal muscle	Present			Expressed in muscles in the head and neck, tongue, back and limbs.

Structure	Level	Pattern	Image	Note
TS24: 4th ventricle	Present	Regionally restricted		Expressed in the ventral ventricular layer.

Structure	Level	Pattern	Image	Note
TS25: 4th ventricle	Present	Regionally restricted		Expressed in the ventral ventricular layer.