Probe:	Mct8 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS26: cochlear ganglion	Weak	Regionally restricted	1B E18	In the spiral ganglion, expression was detected in a neuron-like pattern and partly overlapped with that of Thrb (see assay MGI:5311181).
TS26: cochlear duct	Weak	Regionally restricted	1B E18	Signals were detected in the stria vascularis and at lower levels in the spiral ligament and spiral limbus.
TS26: organ of Corti	Weak	Regionally restricted	1B E18	Signals were detected in the greater epithelial ridge and at lower levels in the lesser epithelial ridge. In the greater epithelial ridge, the expression pattern partly overlapped with that of Thrb (see assay MGI:5311181).

Structure	Level	Pattern	Image	Note
TS27: cochlear ganglion	Present	Regionally restricted	1B P3	In the spiral ganglion, expression was detected in a neuron-like pattern and partly overlapped with that of Thrb (see assay MGI:5311181).
TS27: stria vascularis	Present		1B P3
TS27: spiral ligament	Present		1B P3	Signals were at lower levels in the spiral ligament and spiral limbus
TS27: spiral limbus	Present		1B P3	Signals were at lower levels in the spiral ligament and spiral limbus
TS27: organ of Corti	Present		1B P3	Signals were at lower levels in the lesser epithelial ridge. In the greater epithelial ridge, the expression pattern partly overlapped with that of Thrb (see assay MGI:5311181) and decreased as the tissue was lost between P3 and P15.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	1B P5	In the spiral ganglion, expression was detected in a neuron-like pattern and partly overlapped with that of Thrb (see assay MGI:5311181).
TS28: stria vascularis	Present		1B P5
TS28: spiral ligament	Present		1B P5	Signals were at lower levels in the spiral ligament and spiral limbus.
TS28: spiral limbus	Present		1B P5	Signals were at lower levels in the spiral ligament and spiral limbus.
TS28: organ of Corti	Present		1B P5	Signals were at lower levels in the lesser epithelial ridge. In the greater epithelial ridge, the expression pattern partly overlapped with that of Thrb (see assay MGI:5311181) and decreased as the tissue was lost between P3 and P15.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	1B P7	In the spiral ganglion, expression was detected in a neuron-like pattern and partly overlapped with that of Thrb (see assay MGI:5311181).
TS28: stria vascularis	Present		1B P7
TS28: spiral ligament	Present		1B P7	Signals were at lower levels in the spiral ligament and spiral limbus.
TS28: spiral limbus	Present		1B P7	Signals were at lower levels in the spiral ligament and spiral limbus.
TS28: organ of Corti	Present		1B P7	Signals were at lower levels in the lesser epithelial ridge. In the greater epithelial ridge, the expression pattern partly overlapped with that of Thrb (see assay MGI:5311181).
TS28: cochlear duct	Present		1B P7	Signal strengthened in tympanic border cells at P7-P15.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted	1B P10	In the spiral ganglion, expression was detected in a neuron-like pattern and declined after P7. The expression partly overlapped with that of Thrb (see assay MGI:5311181).
TS28: stria vascularis	Present		1B P10
TS28: spiral ligament	Present		1B P10	Signals were at lower levels in the spiral ligament and spiral limbus.
TS28: spiral limbus	Present		1B P10	Signals were at lower levels in the spiral ligament and spiral limbus.
TS28: organ of Corti	Present		1B P10	Signals were at lower levels in the lesser epithelial ridge. In the greater epithelial ridge, the expression pattern partly overlapped with that of Thrb (see assay MGI:5311181).
TS28: cochlear duct	Present	Regionally restricted	1B P10	Signal strengthened in tympanic border cells at P7-P15.

Structure	Level	Pattern	Image	Note
TS28: cochlear ganglion	Present	Regionally restricted		In the spiral ganglion, the neuron-like expression pattern declined after P7.
TS28: stria vascularis	Present
TS28: spiral ligament	Present
TS28: cochlear duct	Present	Regionally restricted		Signal strengthened in tympanic border cells at P7-P15.

Structure	Level	Pattern	Image	Note
TS27: organ of Corti	Present	Regionally restricted	4A	Signals were detected in the columnar cells of the greater epithelial ridge.

Structure	Level	Pattern	Image	Note
TS28: cochlear duct	Present	Regionally restricted	4C	Signals were detected in the tympanic border cells beneath the sensory epithelium.

Structure	Level	Pattern	Image	Note
TS28: stria vascularis	Present	Regionally restricted	4E	Signals were detected in the intermediate/basal region of stria vascularis.
TS28: spiral ligament	Present	Regionally restricted	4E	Signals were detected in spiral ligament fibrocytes.

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present		6B +/+

Structure	Level	Pattern	Image	Note
TS28: organ of Corti	Present	Regionally restricted	6B Tshr-/-	Signal was elevated in the greater epithelial ridge in the mutant and resembles that of wild type at P3.
TS28: cochlear duct	Present	Regionally restricted	6B Tshr-/-	Signal was reduced in tympanic border cells in the mutant and resembles that of wild type at P3.

Structure	Level	Pattern	Image	Note
TS27: cochlea	Present

Structure	Level	Pattern	Image	Note
TS28: cochlea	Present		S1A Mct8