Antibody:	Anti-PDH E1alpha
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS15: nervous system	Present			Expression was detected in neural tissues.
TS15: heart	Present

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present		8a
TS19: heart	Present		8a
TS19: peripheral nervous system	Present		8a

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present		8b
TS19: heart	Present		8b
TS19: peripheral nervous system	Present		8b

Structure	Level	Pattern	Image	Note
TS19: central nervous system	Present		8c
TS19: peripheral nervous system	Present		8c

Structure	Level	Pattern	Image	Note
TS23: central nervous system	Present		8d
TS23: central nervous system ganglion	Present		8d
TS23: optic nerve	Present		8d
TS23: head	Present	Regionally restricted	8d	Low levels of immunoreactivity were observed in almost all tissues except for some negative cells in cartilages and bones.
TS23: cerebral cortex	Present	Regionally restricted	8d , 8d-I , 8d-II	Expression was abundant in the cortical plate compared to the cortical neuroepithelium.
TS23: cortical plate	Strong		8d , 8d-I

Structure	Level	Pattern	Image	Note
TS19: diencephalon roof plate	Present		8e
TS19: midbrain	Present	Regionally restricted	8e	Expression was detected in the basal region of the mesencephalon.
TS19: metencephalon	Present		8e

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Strong	Regionally restricted	8g	Expression was strong in the Purkinje cells.