Pdha1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:16409 Takakubo F, et al., Analysis of pyruvate dehydrogenase expression in embryonic mouse brain: localization and developmental regulation. Brain Res Dev Brain Res. 1994 Jan 14;77(1):63-76
Assay type:	RT-PCR
MGI Accession ID:	MGI:5308414
Gene symbol:	Pdha1
Gene name:	pyruvate dehydrogenase E1 alpha 1

Probe:	PDH-1, PDH-A
Probe preparation:	labelled with P32
Visualized with:	Autoradiography
Assay notes:	A graph of the developmental profile was shown in Fig. 7a.

Results

Image: 4_pdha1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	273 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
M	Mol. Wt. Marker Lane
1	E9.5 (b)	TS15: future brain	Present (c)	5 µg; total RNA	Arc:ARC(S)		Not Specified
2	E11.5 (b)	TS19: brain	Present	5 µg; total RNA	Arc:ARC(S)		Not Specified
3	E15.5 (b)	TS23: brain	Present	5 µg; total RNA	Arc:ARC(S)		Not Specified
4	E18.5 (b)	TS26: brain	Present	5 µg; total RNA	Arc:ARC(S)		Not Specified
5	P adult	TS28: brain	Present	5 µg; total RNA	Arc:ARC(S)		Not Specified
6	P adult	TS28: heart	Present (d)	5 µg; total RNA	Arc:ARC(S)		Not Specified
7	P adult	TS28: metanephros	Present (d)	5 µg; total RNA	Arc:ARC(S)		Not Specified

Notes:
(a) Expression level gradually decreased between E9.5 and E15.5, then a dramatic increase took place between E15.5 and E18.5.
(b) Age normalized so that noon of plug day = E0.5.
(c) Expression in E9.5 brain was approximately 93% of the adult brain value.
(d) Present assigned by curator.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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