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Gene Expression Data
Assay Details
Assay
Reference: J:179954 Matsumoto S, et al., Expression analysis of the regenerating gene (Reg) family members Reg-IIIbeta and Reg-IIIgamma in the mouse during development. J Comp Neurol. 2012 Feb 15;520(3):479-94
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5308276
Gene symbol: Reg3g
Gene name: regenerating islet-derived 3 gamma
Antibody: anti-Reg-IIIgamma
Detection system: Secondary antibody coupled to Alexa Fluor
Assay notes: Transcardial perfusion with 2% or 4% paraformaldehyde.
Results
Specimen 4H: postnatal week 7 (more )
Note: Double labeled: green - Reg3g; magenta - Omp (assay MGI:5308277).
Structure Level Pattern Image Note
TS28: nasal cavity respiratory epithelium Present Regionally restricted 4H Not co-localized with marker for olfactory epithelial cells.
Specimen 4I: postnatal week 7 (more )
Note: Double labeled: green - Reg3g; magenta - Foxj1 (assay MGI:5308278).
Structure Level Pattern Image Note
TS28: nasal cavity respiratory epithelium Present Regionally restricted 4I Not co-localized with marker for ciliated cells.
Specimen 4J: postnatal week 7 (more )
Note: Double labeled: green - Reg3g; magenta - Muc5ac (assay MGI:5308279).
Structure Level Pattern Image Note
TS28: nasal cavity respiratory epithelium Present Regionally restricted 4J Co-localized with marker for goblet cells at the apical compartment containing secretory proteins.
Specimen 5G: postnatal week 7 (more )
Note: Double labeled: green - Reg3g; magenta - Foxj1 (assay MGI:5308278).
Structure Level Pattern Image Note
TS28: trachea epithelium Present Regionally restricted 5G Not co-localized with marker for ciliated cells.
Specimen 5H: postnatal week 7 (more )
Note: Double labeled: green - Reg3g; magenta - Muc5ac (assay MGI:5308279).
Structure Level Pattern Image Note
TS28: trachea epithelium Present Regionally restricted 5H Co-localized with marker for goblet cells at the apical compartment.
Specimen 5I: postnatal week 7 (more )
Note: Double labeled: green - Reg3g; magenta - Scgb1a1 (assay MGI:5308280).
Structure Level Pattern Image Note
TS28: trachea epithelium Present Regionally restricted 5I Co-localized with marker for nonciliated epithelial cells (Clara-like cells) at the apical compartment.
Specimen 7N: postnatal week 7 (more )
Structure Level Pattern Image Note
TS28: intestine epithelium Present Regionally restricted 7N Expressed in the bottom of the crypt (Paneth cells).
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory