Thbs2 RNase protection Gene Expression Assay - GXD

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Gene Expression Data
Assay Details

Assay

Reference:	J:63332 Melnick M, et al., Thrombospondin-2 gene expression and protein localization during embryonic mouse palate development. Arch Oral Biol. 2000 Jan;45(1):19-25
Assay type:	RNase protection
MGI Accession ID:	MGI:5307155
Gene symbol:	Thbs2
Gene name:	thrombospondin 2

Probe:	Thbs2 cDNA4
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Phosphorimaging
Assay notes:	Each sample was a pool of 5-8 palates from one litter.

Results

Image: 1A

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	400 b	Amount	Genetic Background	Mutant Allele(s)	Sex
E13	E13.5 (a)	TS21: palatal shelf	Present	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E13	E13.5 (a)	TS21: palatal shelf	Present	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E13	E13.5 (a)	TS21: palatal shelf	Present	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E14	E14.5 (a)	TS22: palatal shelf	Present	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E14	E14.5 (a)	TS22: palatal shelf	Present	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E14	E14.5 (a)	TS22: palatal shelf	Present	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E15	E15.5 (a)	TS23: palatal shelf	Present (b)	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E15	E15.5 (a)	TS23: palatal shelf	Present (b)	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified
E15	Control
E15	E15.5 (a)	TS23: palatal shelf	Present (b)	Not Specified	B10.A-H2^a H2-T18^a/SgSnJ		Not Specified

Notes:
(a) Age normalized so that noon of plug day = E0.5.
(b) There is a 31% decline with maturation.

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