Tg(Lck-Ets2delta)1Boul
Transgene Detail
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| Symbol: |
Tg(Lck-Ets2delta)1Boul |
| Name: |
transgene insertion 1, Kim E Boulukos |
| MGI ID: |
MGI:5306588 |
| Synonyms: |
lckPr-deltaets2 |
| Transgene: |
Tg(Lck-Ets2delta)1Boul Location: unknown
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| Alliance: |
Tg(Lck-Ets2delta)1Boul page
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| Strain of Origin: |
C57BL/6 x DBA
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| Transgene Type: |
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Transgenic (Dominant negative, Inserted expressed sequence) |
| Mutation: |
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Insertion
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Mutation details: This transgene contains 1.1 kb of the 2.1 kb human v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) (ETS2) cDNA inserted into the p1017 expression vector between the T cell-specific, proximal promoter of the mouse lymphocyte protein tyrosine kinase (Lck) gene and the human growth hormone (GH1) genomic sequence, including the GH1 polyadenylation signal. The truncated ETS2 encoded by the transgene corresponds to the DNA binding domain of the normal protein; it competes with the endogenous mouse ETS2 and thus acts as a dominant negative mutant. Based on Southern blot analysis, 20 copies of the transgene are estimated to be present. Western blot analysis of total thymocyte lysates from these mice using anti-ETS2 antibodies demonstrates expression of the truncated ETS2 protein.
(J:79809)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 1 strain available
Cell Lines: 0 lines available
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| Original: |
J:79809 Zaldumbide A, et al., The role of the ets2 transcription factor in the proliferation, maturation, and survival of mouse thymocytes. J Immunol. 2002 Nov 1;169(9):4873-81 |
| All: |
1 reference(s) |
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Analysis Tools
