Ptf1a Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:50644 Krapp A, et al., The p48 DNA-binding subunit of transcription factor PTF1 is a new exocrine pancreas-specific basic helix-loop-helix protein. EMBO J. 1996 Aug 15;15(16):4317-29
Assay type:	Northern blot
MGI Accession ID:	MGI:5305193
Gene symbol:	Ptf1a
Gene name:	pancreas specific transcription factor, 1a

Probe:	p48 cDNA2
Probe preparation:	labelled with P32 DNA
Visualized with:	Phosphorimaging

Results

Image: 6B

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
13	E13.0 (a)	TS21: pancreas	Not Specified (b)	1.0 µg; poly-A+ RNA	BALB/c		Not Specified	Excised pancreatic tissue included mostly rudiments of gut and stomach
14	E14.0 (a)	TS22: pancreas	Not Specified (b)	1.0 µg; poly-A+ RNA	BALB/c		Not Specified	Excised pancreatic tissue included mostly rudiments of gut and stomach
15	E15.0 (a)	TS23: pancreas	Absent	1.0 µg; poly-A+ RNA	BALB/c		Not Specified
16	E16.0 (a)	TS24: pancreas	Present (c)	1.0 µg; poly-A+ RNA	BALB/c		Not Specified
17	E17.0 (a)	TS25: pancreas	Present (c)	1.0 µg; poly-A+ RNA	BALB/c		Not Specified
18	E18.0 (a)	TS26: pancreas	Present (c)	1.0 µg; poly-A+ RNA	BALB/c		Not Specified
Ad	P adult	TS28: pancreas	Present	0.1 µg; poly-A+ RNA	BALB/c		Not Specified
16	E16.0 (a)	TS24: pancreas	Present (c)	1.0 µg; poly-A+ RNA	BALB/c		Not Specified	Sample RNA was depolyadenylated.
Ad	P adult	TS28: pancreas	Present	0.1 µg; poly-A+ RNA	BALB/c		Not Specified	Sample RNA was depolyadenylated.

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Authors report expression at stage prior to day 15 of gestation, but they state that the relative abundance of early RNAs cannot be evaluated.
(c) Embryos expression RNA of higher molecular weight in addition to those found in adult pancreas.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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