Cdh5tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst
Targeted Allele Detail
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| Symbol: |
Cdh5tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst |
| Name: |
cadherin 5; targeted mutation 4, Dietmar Vestweber |
| MGI ID: |
MGI:5305106 |
| Synonyms: |
VE-cadherin FKBP/VE-PTP FRB* |
| Gene: |
Cdh5 Location: Chr8:104828257-104871143 bp, + strand Genetic Position: Chr8, 53.04 cM
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| Alliance: |
Cdh5tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:178759
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| Parent Cell Line: |
Not Specified (ES Cell)
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| Strain of Origin: |
Not Specified
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| Allele Type: |
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Targeted (Inserted expressed sequence, RMCE-ready) |
| Mutations: |
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Insertion, Intragenic deletion
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Cdh5tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst expresses
4 genes
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Mutation details: A floxed cassette containing the rapamycin binding domain of the FK 506 binding protein fused to the C terminus of Cdh5, an IRES, a mutated form of the rapamycin binding domain of mammalian target of rapamycin (FRB*), containing an HA-tag at its C terminus, fused to the C terminus of Ptprb, and an FRT flanked hygromycin gene was inserted via recombinase mediated cassette exchange using Cdh5tm1Dvst. In lungs from homozygous mice wild-type protein is not expressed and expression of the mutant Cdh5/Fkbp protein is detected at levels similar to wild-type protein in control mice. Expression of the mutant Ptprb/Mtor* protein is about 30% of the level of expression of the endogenous protein in lungs from homozygous mice. Dissociation of heterodimers of the two mutant proteins is impaired in the presence of rapalog.
(J:178759)
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Generation of the Cdh5tm4(Cdh5/Fkbp,Ptprb/Mtor*)Dvst allele |
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Cdh5 Mutation: |
54 strains or lines available
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| Original: |
J:178759 Broermann A, et al., Dissociation of VE-PTP from VE-cadherin is required for leukocyte extravasation and for VEGF-induced vascular permeability in vivo. J Exp Med. 2011 Nov 21;208(12):2393-401 |
| All: |
3 reference(s) |
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Analysis Tools
