Scgb3a1 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:82365 Reynolds SD, et al., Secretoglobins SCGB3A1 and SCGB3A2 define secretory cell subsets in mouse and human airways. Am J Respir Crit Care Med. 2002 Dec 1;166(11):1498-509
Assay type:	RNA in situ
MGI Accession ID:	MGI:5304823
Gene symbol:	Scgb3a1
Gene name:	secretoglobin, family 3A, member 1

Probe:	IMAGE clone 318938
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Results

Specimen 2B: postnatal month 2-4 (more

)

Structure	Level	Pattern	Image	Note
TS28: bronchus epithelium	Present	Regionally restricted	2B	Expressed predominantly in bronchi.
TS28: bronchiole epithelium	Absent		2B	Little if any expression in terminal bronchioles.

Specimen 3A: postnatal month 2-4 (more

)
Note: This is from the dorsal trachea.

Structure	Level	Pattern	Image	Note
TS28: trachea epithelium	Present	Regionally restricted	3A	Expressed at a higher level than Scgb3a2 (see assay MGI:5304837). Expression is greater in ventral relative to dorsal trachea.

Specimen 3B: postnatal month 2-4 (more

)
Note: This is from the ventral trachea.

Structure	Level	Pattern	Image	Note
TS28: trachea epithelium	Present	Regionally restricted	3B	Expression is greater in ventral relative to dorsal trachea, and increases along the proximal to distal axis of the airway in a shallow gradient.

Specimen 3C: postnatal month 2-4 (more

)

Structure	Level	Pattern	Image	Note
TS28: trachea submucosa	Present	Regionally restricted	3C	Expression in the epithelial cells lining submucosal glands.
TS28: trachea epithelium	Present	Regionally restricted	3C	Enriched in the intercartilaginous regions at some levels.

Specimen 3D: postnatal month 2-4 (more

)

Structure	Level	Pattern	Image	Note
TS28: trachea submucosa	Present	Regionally restricted	3D	Expression in the epithelial cells lining submucosal glands.
TS28: trachea epithelium	Present	Regionally restricted	3D	Enriched in the intercartilaginous regions at some levels.

Specimen 5B: embryonic day 18.5 (more

)

Structure	Level	Pattern	Image	Note
TS26: trachea epithelium	Strong	Regionally restricted	5B
TS26: bronchiole epithelium	Absent		5B	Little or no expression in the intralobar region of the conducting airway epithelium.
TS26: bronchus epithelium	Absent		5B	Little or no expression in the intralobar region of the conducting airway epithelium.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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