Gene Expression Data
Assay Details
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Assay
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Reference:
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J:80025
Wallis D, et al., The zinc finger transcription factor Gfi1, implicated in lymphomagenesis, is required for inner ear hair cell differentiation and survival. Development. 2003 Jan;130(1):221-32
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5302559
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Gene symbol:
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Casp3
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Gene name:
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caspase 3
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Antibody:
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anti-activated Caspase 3 (AF835)
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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The antibody used in this assay recognizes the activated form of the protein.
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Results
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Specimen
6E:
postnatal day 7
(more  )
Specimen
6E:
(close  )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Gfi1tm1Sho/Gfi1tm1Sho
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Age:
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postnatal day 7
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear ganglion |
Present |
Single cells |
6F
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As in wild type, low levels of apoptosis are observed.
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Specimen
6G:
postnatal day 21
(more )
Specimen
6G:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129S1/Sv * C57BL/6J
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Mutant Allele(s):
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Gfi1tm1Sho/Gfi1tm1Sho
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Age:
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postnatal day 21
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cochlear ganglion |
Present |
Single cells |
6H
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The levels of apoptosis observed are higher than those in wild type. Also, cell density is slightly lower compared to wild type.
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Analysis Tools
